摘要
目的:建立测定雷贝拉唑钠血药浓度的高效液相色谱法。方法:采用Hypersil C18柱(4.6mm×200mm,5μm),以乙腈-水(32∶68)为流动相,流速1.0ml/min,检测波长288nm。结果:本方法线性范围5～1600μg/L,r=0.9993,回收率为95.48%～99.13%。日内及日间RSD均小于4%。结论:本方法灵敏、准确、重现性好,适用于雷贝拉唑钠药代动力学研究。
Objective：To set up a reverse phase HPLC method for determining the concentration of rabeprazole sodium in serum in order to study its clinical pharmacokinetics.Methods：The chromatographic separation was carried out on Hypersil C18 column（4.6 mm×200 mm,5 μm）, the mobile phase was acetonitrile-water（32：68） and flow rate was 1.0 ml/min. The detector wavelength was 288 nm.Results：The linear range was 5-1 600 μg/L, r=0.999 3. The method recovery was within 95.48%-99,13%,within-day and between-day R SD were less than 4%.Conclusion ：The method is sensitive, specific and reproducible for the determination of rabeprazole sodium concentration in humam plasma.It is very suitable for pharmacokinetics study of rabeprazole sodium.
出处
《中国医药导报》
CAS
2008年第3期29-30,共2页
China Medical Herald
