摘要
目的 建立HPLC—MS—MS测定人尿液中黄芪甲苷的浓度。方法 使用C18（4．6mm×100mm，5μm）色谱分析枉，以98％CH3OH为流动相，流速0．3mL·min^-1尿液样品经甲醇沉淀后进样，选择黄芪甲苷离子对（807．3／627．3m／z）为选择性检测离子，按外标法定量。结果 尿液中黄芪甲苷线性范围为0．0125～2．0mg·L^-1，日内和日间RSD均小于6％，方法回收率在97．5％-104．2％，最低定量质量浓度为0．0125mg·L^-1。结论 本方法简便、快速、特异，适用于黄芪甲苷人体药动学研究。
OBJECTIVE To establish a sensitive and specific HPLC-MS-MS method for the determination of Astragaloside Ⅳ in human urine. METHODS The Kromasil C18 （4. 6 mm × 100 mm,5 μm）analytic column was used. 98% Methanol was used as mobile phase at the flow rate of 0. 3 mL . min^-1. Detection was performed on API mass spectrometry equipped with an ESI and operated in positive-ionization mode. Astragaloside quantitation in urine was calculated by computing the peak area of Astragaloside Ⅳ （ 807.3/ 627. 3 ）. RESULTS The calibration curve was linear over the range of 0. 012 5 - 2. 0 mg . L^ -1 in human urine, and the within-day and between-day precisions were less than 6% o The method recovery was 97.5% - 104.2%. CONCLUSION HPLC-MS-MS Method established in this study is simple, sensitive with precision, which can be adapted to pharmacokinetic study.
出处
《中国药学杂志》
CAS
CSCD
北大核心
2008年第1期55-57,共3页
Chinese Pharmaceutical Journal
