摘要
本文采用三种方法:滋养培养、花粉粒离体培养、微室培养对三种转基因番茄花粉进行组织培养,诱导纯合二倍体。花粉粒离体培养诱导愈伤组织或胚状体的效率高于滋养培养。微室培养可在镜下直接观测花粉粒发育情况。采用不同花药预处理方法,可提高诱导效率。
To breed three kinds of transgenic tomatoes into pure diploid,three methods:nurse cul- ture,pollen grain culture in vitro,microchamber culture was used in this paper.The efficiency of inducing calli or pollen embryos using pollen grain culture in vitro is higher than using nurse culture.The development of the pollen grain can be observed directly in micro-chamber cul- ture.Different method of poUen prepmcessing was used to raise the callus-inducing rate.
出处
《哈尔滨师范大学自然科学学报》
CAS
1997年第2期109-111,共3页
Natural Science Journal of Harbin Normal University
基金
国家自然科学基金
