摘要
目的:探讨普鲁卡因(procaine)对人鼻咽癌(NPC)细胞CNE-2Z的影响。方法:体外培养的NPC细胞CNE-2Z分别加入不同浓度的普鲁卡因,并作用不同的时间。应用四甲基偶氮唑盐[3-(4,5-Dimethyl-thiaoly)-2,5-diphenyl-2H tetrazolium bromide,MTT]法和流式细胞仪检测观察普鲁卡因对NPC细胞CNE-2Z的影响,同时用RT-PCR分析p16和RASSF1A mRNA的表达情况。结果:普鲁卡因能显著抑制CNE-2Z细胞增殖,该作用呈剂量-效应和时间依赖关系。普鲁卡因使细胞周期阻滞于G1期,并能上调CNE-2Z细胞RASSF1A mRNA的表达,但对p16 mRNA表达无明显影响。结论:普鲁卡因对CNE-2Z细胞有增殖抑制作用,它能上调CNE-2Z细胞RASSF1A mRNA的表达,推测这可能是普鲁卡因抑制CNE-2Z细胞增殖的重要分子机制之一。
Objective：To study the effects of procaine on human nasopharyngeal carcinoma cell strain CNE- 2Z. Method..CNE-2Z cells-cultured in vitro-were exposed to different concentration procaine and different time. Its growth characteristic were studied by using MTT assay and flow cytometry. The expresstion of p16 and RASSF1A mRNA were analyzed by RT-PCR. Result.. Procaine could inhibit the proliferation of CNE-2Z cells and the effects were in doso-and time-dependent manners. Procaine also arrested CNE-2Z cells in G1 phase of cell cycle. The expression of RASSF1A mRNA was up-regulated in CNE-2Z cells treated with procaine, but no obvious change was found in the expression of p16 mRNA. Conclusion ： Procaine could inhibit cell growth and up-regulate the expression of RASSF1A mRNA, which may play an important role in CNE-2Z cells＂ proliferation inhibition induced by procaine.
出处
《临床耳鼻咽喉头颈外科杂志》
CAS
CSCD
北大核心
2007年第24期1118-1121,共4页
Journal of Clinical Otorhinolaryngology Head And Neck Surgery
