摘要
目的:观察CD40-CD40配体(CD40-CD40L)相互作用对体外培养的大鼠主动脉平滑肌细胞(VSMC)增殖及迁移的影响。方法:应用组织贴块法进行SD大鼠主动脉VSMC原代培养,以3H-胸腺嘧啶脱氧核苷(3H-TdR)、3H-亮氨酸(3H-Leu)掺入法分别测定VSMC增殖,VSMC的迁移采用琼脂糖凝胶刮取法,用倒置显微镜观察。结果:CD40-CD40L相互作用能明显促进VSMC3H-TdR、3H-Leu向细胞的掺入率,两者具有时间、剂量依赖性,CD40-CD40L相互作用随CD40L浓度的增加及刺激时间延长(30h内),能明显增加VSMC迁移率,抗-CD40单克隆抗体能明显抑制上述效应。结论:CD40-CD40L相互作用能明显促进主动脉VSMC增殖和迁移。
Objective：To investigate the effect of CD40-CD40 ligand interaction on the proliferation and migration in cultured aortic vessel smooth muscle cells （VSMC） of rat. Method： The primary passage smooth muscle cells of rats were cultured by tissue mince-sticking method and the 3rd and 4th passage cells were incubated with indicated concentrations of CD40 ligand （CD40L） for various periods. The incorporation of ^3H-TdR and ^3H-Leu analysis was used to evaluate the cell proliferation. Cell migration was obtained by monolayer cell scrape and observed by reverse microscope. Result：The incorporation of ^3H-TdR, ^3H-Leu and the migration of smooth muscle cells were significantly increased by CD40L with a dose- and time-dependent manner. Anti-CD40 monoclonal antibody significantly suppressed CD40L-induced incorporation of ^3H-TdR, ^3H-Leu and the migration of VSMC. Conclusion： The interaction of CD40-CD40L could promote the proliferation and migration of cultured aortic VSMC of rat.
出处
《临床心血管病杂志》
CAS
CSCD
北大核心
2007年第11期854-857,共4页
Journal of Clinical Cardiology
基金
江苏省自然基金资助(No:BK2005069)
关键词
抗原
CD40
CD40配体
肌
平滑
血管
增殖
Antigens, CD40
CD40 ligand
Muscle, smooth, vascular
Proliferation
