摘要
利用AFLP技术,选择EcoRⅠ/MesⅠ这一酶切组合,应用18对EcoRⅠ+3/MesⅠ+3引物组合进行选择性扩增,检测10个马蔺种群的基因组DNA多态性。共扩增出1 164个遗传位点,其中752个多态位点,多态率为65.11%。并通过Jaccard的方法将电泳带矩阵转化为遗传相似性系数矩阵,进行UPGMA聚类分析。结果表明,当取阈值为0.74时,可以将10个种群分为4大类群。长春马蔺与其他种源的亲缘关系较远,单独聚为一类;宁夏固原、甘肃民勤、武威、北京、山东泰山5个种群亲缘关系较近,聚为一类;内蒙古太仆寺和西乌旗、新疆乌鲁木齐聚为一类;河北涿洲马蔺种群单独聚为一类。马蔺群体间的亲缘关系远近与其所处的地理位置有很大的关系,尤其与纬度因子的关系十分密切。
The AFLP （amplified fragment length polymorphism） technique was used to analyze genetic diversity of 10 different populations of Iris lactea collected from China. Genomic DNA was digested with EcoR Ⅰ and Mse Ⅰ enzymes and amplified with 18 pairs of EcoR Ⅰ ＋ 3/Mes Ⅰ ＋ 3 Primers combinations. AFLP analysis produced 1 164 scoreable bands,of which 752 （65. 11% ） were polymorphic. Cluster Analysis was based on the UPGMA method. Populations in Changchun and Zhuozhou city showed a greater genetic variability and were separately classified into two groups. Populations in Guyuan, Minqin, Wuwei, Beijing and Taishan were classified into one group, and those in Taipu, Xiwuqi and Wulumuqi were in another group. The genetic relationships among the populations were related to their locations, especially to the latitude.
出处
《安徽农业大学学报》
CAS
CSCD
北大核心
2008年第1期95-98,共4页
Journal of Anhui Agricultural University
基金
国家重大科技基础性工作专项(2002DEAl0009)资助
