摘要
以棉枯萎镰刀菌菌丝体加孢子提取的蛋白质为抗原免疫家兔制备的２种抗血清Ａｓ２、Ａｓ１０中成分复杂，用琼脂双扩散法和比较对流免疫电泳法测定特异性，交叉反应明显。Ａｓ２用木贼镰刀菌的异种抗原、Ａｓ１０用禾谷镰刀菌的异种抗原吸收排除非特异性成分后，抗血清的专化性提高，消除了交叉反应。特异性测定结果表明：利用此种经吸收后的抗血清检测棉枯萎镰刀菌可以准确地鉴定到尖孢镰刀菌种的水平，但不能区分专化型。
In this study, the soluble proteins used as antigen were extracted from the mycellia and conidia of the pathogen Fusarium oxysporum f. sp. vasinfectum of cotton fusarium wilt, and the antisera (As2, As10) obtained by immunizing the rabbits contained complicated composition. The nonspecific reaction affected the serodedected result by agar dimension diffusion (ADD) and comparative immunoosmopheresis (CIOP). By mixing As2 and the antigen Fusarium equiseti , As10 and the antigen Fusarium graminearum , then eliminating the nonspecific composition, the specificity of antisera was improved. The results of this study revealed that As2 and As10 can detect the species of Fusarium oxysprorum , but can′t detect forme species.
出处
《华中农业大学学报》
CAS
CSCD
北大核心
1997年第4期343-346,共4页
Journal of Huazhong Agricultural University
关键词
枯萎菌
血清学
琼脂双扩散
棉花
Fusarium oxysporum , serology, agar dimension diffusion (ADD), comparative immunosmopheresis (CIOP)
