摘要
为探讨大鼠坐骨神经损伤后脊髓小胶质细胞反应性、脊髓腹角运动神经元脱失与坐骨神经再生之间的关系,制备了SD大鼠右侧坐骨神经钳夹损伤模型,术后3d和7d测定相应脊髓节段小胶质细胞免疫反应性、腹角运动神经元数量,4周时于光镜和电镜下评价坐骨神经变性和再生。结果显示:(1)坐骨神经损伤后3d,脊髓腹角小胶质细胞OX-42免疫反应性开始明显增强(P<0.05);(2)脊髓腹角损伤同侧与对侧运动神经元数量比明显降低(P<0.05),说明同侧运动神经元存活数量减少;(3)组织学评价显示损伤神经再生不良;(4)simvastatin(一种降胆固醇药物,具有潜在的免疫调节作用)干预组较非simvastatin干预组小胶质细胞进一步激活,运动神经元存活数量增加,坐骨神经再生良好。本研究结果提示,脊髓腹角小胶质细胞的激活可能在大鼠周围神经损伤后的再生中发挥重要的保护作用。
To explore the relationship of microglia activation, motoneuronal loss in the ventral hom of spinal cord and sciatic nerve regeneration after the sciatic nerve injury, Sprague-Dawley rats were prepared a medal of the right sciatic nerve crush injury. The immunoreactivity （-ir） of microglia and number of the motoneurons in the ventral horn of spinal cord were detected at 3 and 7 days, light and electron microscopic detection of sciatic nerve degeneration and regeneration were performed at 4 weeks after the nerve injury. The results showed： （ 1 ） At 3 days after the sciatic nerve injury, OX-42-ir in the ventral horn of spinal cord began to increase significantly （ P 〈 0.05 ） ; （ 2 ） The number ratio of motoneurons in ventral horn of spinal cord in ipsilateral to contralateral for injury decreased markedly （ P 〈0.05 ）, indicating the numbers of ipsilateral motoneuronal survival decreased ; （ 3 ） Histological assessment showed the poor regeneration of the injured nerves; （4） Simvastatin （ an inhibitor of cholesterol biosynthesis, having potential immunomedulatory capacities） facilitated the microglial activation, the motoneuronal survival and sciatic nerve regeneration were better than non-simvastatin-treated vehicle rats. The present results suggest that microglia activation in the ventral horns of the spinal cord may play an important protective role in the nerve regeneration after peripheral nerve injury of the rat.
出处
《神经解剖学杂志》
CAS
CSCD
北大核心
2008年第1期31-36,共6页
Chinese Journal of Neuroanatomy
