摘要
目的建立早孕妇女血和胚胎组织中氟康唑浓度测定的RP-HPLC法。方法血和胚胎组织样品采用1 mol·L^(-1) NaOH溶液碱化、乙酸乙酯萃取后行HPLC测定。色谱柱:Diamonsil^(TM)C_(18)(200 mm×4.6 mm,5μm),流动相:乙腈-0.03 mol·L^(-1)磷酸盐缓冲液(pH 5.8,25:75,V/V),流速1.2 mL·min^(-1);检测波长260 nm;柱温40℃。结果血、胚胎组织样品分别在0.2～10 mg·L^(-1)和0.133～6.667μg·g^(-1)浓度范围内线性良好,最低检测浓度分别为0.1 mg·L^(-1)和0.066 7μg·g^(-1),方法回收率为96.56～104.35%,日内RSD≤6.81%,日间RSD≤4.78%,血和胚胎组织中氟康唑的萃取回收率均>70%。结论此法能简便、灵敏、准确地测定早孕妇女血和胚胎组织中氟康唑的浓度,可用于临床药动学研究。
AIM To develop a reversed phase HPLC method for the determination of fluconazole level in pregnant women plasma and embryo tissue. METHODS The plasma and embryo tissue samples were alkalinized with 1 mol· L^- 1 NaOH and extracted with acetoacetate. Chromatographic separation was achieved at 40℃; on a Diamonsil^TM C18 （ 200 mm × 4.6 mm, 5 μm） analytical column. The mobile phase was acetonitrile - 0.03 mol· L^-1 phosphate buffer solution （pH 5.8,25：75, V/V）. The flow rate was 1.2 mL·min^-1. UV detection was set at 260 nm. RESULTS The linear range of fluconazole was 0.2 - 10 mg·L^-1 and 0. 133 - 6.667 μg·g^-1 and the detection limits were 0.1 mg·L^-1 and 0.066 7 μg· g^-1 for plasma and embryo tissue samples, respectively. Method recovery was 96.56% - 104.35%. Intraand inter-day variations were less than 6.81% and 4.78%, respectively. The extraction recoveries of fluconazole from plasma and tissue homogenates were more than 70%. CONCLUSION The established method is a simple, sensitive and accurate means for determining fluconazole level in pregnant women plasma and embryo tissue, and it is suitable for clinical phamaacokinetic studies.
出处
《中国临床药学杂志》
CAS
2008年第1期44-47,共4页
Chinese Journal of Clinical Pharmacy
基金
深圳市福田区科技局(FT200518号)资助
