摘要
目的探讨1，25-二羟基维生素D3（1，25（OH）2D3）对原发性骨质疏松骨折愈合的影响。方法采用8月龄雌性新西兰兔，摘除双侧卵巢法建立Ⅰ型骨质疏松模型，随机分为假手术组（Sham）、单纯去卵巢组（OVX）、葡萄糖酸钙组（OC）和葡萄糖酸钙＋1，25（OH）2D3组（OCR）。采用3岁龄新西兰雌兔和4岁龄新西兰雄兔，作为Ⅱ型骨质疏松动物模型，随机分为空白对照组（Control）、葡萄糖酸钙组（Calcium）、葡萄糖酸钙＋1，25（OH）2D3组（CR）。所有实验动物行双侧桡骨骨折模型。OC组和Calcium组单纯给予葡萄糖酸钙，OCR组和CR组给予葡萄糖酸钙和1，25（OH）2D3。于给药后2、4、8周给各实验组拍骨折X线片，8周测定各实验组骨痂、腰椎的骨密度（BMD），对骨痂组织进行组织学观察。结果给药8周后，OCR组的X线评分、腰椎和骨痂骨密度均较OVX组和OC组显著升高（P〈0.05或P〈0.01）。CR组的X线评分、腰椎和骨痂骨密度均较Control组和Calcium组显著升高（P〈0.05或P〈0.01）。结论1，25（OH）2D3对Ⅰ型和Ⅱ型骨质疏松骨折愈合均有促进作用。
Objective To investigate the effects of 1,25-dihydroxyvitamin D3 on the fracture healing of primary osteoporosis. Methods Female New Zealand white rabbits aged 8 months were bilaterally ovariectomized as models of postmenopausat osteoporosis. Then they were random divided into 4 groups： pseudo-ovariectomized group（Sham）, ovariectomized group （OVX）, calcii gluconas group （OC）, calcii gluconas and 1,25-dihydroxyvitamin D3 group （OCR）. Both female New Zealand white rabbits aged 3 years and male New Zealand white rabbits aged 4 years were selected as models of senile osteoporosis and random divided into 3 groups： control group （ Control）, calcii gluconas group （Calcium）, calcii gluconas and 1,25-dihydroxyvitamin D3 group （CR）. Then the models of both sides＇radius fractures were made among all , the experimental animals. Both OC group and Calcium group were given calcii gluconas. Both OCR group and CR group were given calcii gluconas and 1,25-dihydroxyvitamin D3. The X-photograph of fractures were taken among all the experimental animals after they were given drug for 2, 4, 8 weeks. All the fracture specimens were taken from the experimental animals after given drug for 8 weeks. Then the bone mineral density （ BMD ） of the bony callus and lumbar vertebrates of all experimental animals were determined and the bony calluses were observed. Result After the experimental animals＇were given drug for 8 weeks, the X-ray score, BMD of lumbar vertebrates and bony callus of OCR group were significantly higher than that in OVX group and OC group （ P 〈 0. 05 or P 〈 0. 01 ）. The X-ray score, BMD of lumbar vertebrates and bony callus of female and male CR groups were significantly higher than that in control groups and Calcium groups （ P 〈0. 05 or P 〈0. 01 ）. Conclusion 1,25-dihydroxyvitamin D3 can accelerate fracture healing in postmenopausal osteoporosis and senile osteoporosis.
出处
《中国医师杂志》
CAS
2007年第12期1604-1607,共4页
Journal of Chinese Physician
