摘要
目的:研究黄芩甙对人肝癌BEL-7402细胞系黏附、细胞凋亡及细胞周期的影响,探讨三者之间的关系.方法:应用细胞培养技术培养BEL-7402细胞,不同浓度黄芩甙处理肝癌细胞,通过Boyden小室模型测定其黏附力,同时观察细胞形态,流式细胞术测定肝癌细胞integrinβ_1、E-cd表达的改变及细胞凋亡率.结果:5,10,20mg/L黄芩甙处理组细胞黏附力与对照组相比均有显著降低(52.38±3.83,35.63±2.32,26.58±1.13 vs 84.32±6.74.p<0.05).对照组表现出纤维母细胞样形态,伸出形态各异的伪足.而黄芩甙组细胞形态较圆,伪足数目相对较少,电镜下显示细胞微绒毛明显减少,甚至消失.5,10,20mg/L黄芩甙组细胞integrinβ_1表达较对照组降低(354.07±18.17,319.44±15.21,291.49±12.88 vs 407.54±20.58:P<0.05),E-cd的表达则增加(335.5±20.18,376.4±22.36,393.8±25.68 vs 251.72±12.34,P<0.05).黄芩甙作用后,随黄芩甙浓度增加肝癌细胞G_0/G_1,期比例逐步增高,S期细胞减少,同时细胞凋亡率增加.结论:黄芩甙对肝癌BEL-7402细胞发挥抗黏附作用,其机制可能与抑制黏附分子E-cd的表达,促进integrinβ_1表达有关.在抗黏附的同时细胞周期发生改变,细胞凋亡增多.
AIM： To investigate the effect of baicalin on adhesion, apoptosis and cell cycle of hepatocellular carcinoma （HCC） cell line BEL-7402, and to discuss the relationship between them.
METHODS： HCC cell line BEL-7402 adhesion assay was conducted in a Boyden chamber. Expression of integrin J31 and E-cd proteins of cell line BEL-7402 was determined by flow cytometry （FCM）. The effects of baicalin on cell cycle and apoptosis were also observed by FCM. Changes in morphology and ultrastructure were observed by inverted microscopy and electron microscopy.
RESULTS： The adhesion capacity was 52.38 ± 3.83, 35.63 ± 2.32 and 26.58 ± 1.13 at 5, 10 and 20 mg/L baicalin, respectively, and was lower than that in the control group （84.32 ± 6.74; P 〈 0.05）. In the treated group, the HCC cells became smaller and remained round with relatively fewer pseudopods, and microvilli were fewer and shorter. After treatment with 5, 10 and 20 mg/L baicalin, expression of integrin β1 was decreased （354.07 ± 18.17, 319.44 ± 15.21 and 291.49 ± 12.88 vs 407.54 ± 20.58; P 〈 0.05）, and expression of E-cd was increased significantly （335.5 ± 20.18, 376.4 ± 22.36 and 393.8 ± 25.68 vs 251.72 ± 12.34, P 〈 0.05）. G0/G1 phase cells were significantly increased as baicalin concentration increased, and S phase cells were decreased. Baicalin induced BEL-7402 cell apoptosis, in a concentration- dependent manner.
CONCLUSION： Baicalin inhibits cell adhesion, accompanied by increased apoptosis and major changes in the cell cycle of HCC cell line BEL-7402.
出处
《世界华人消化杂志》
CAS
北大核心
2007年第32期3390-3394,共5页
World Chinese Journal of Digestology
关键词
黄芩甙
肝细胞癌
黏附
凋亡
细胞周期
Baicalin
Hepatocellular carcinoma
Adhesion
Apoptosis
Cell cycle
