摘要
目的以PLGA和胶原海绵为载体,分别复合rhBMP-2,比较两者修复兔关节软骨缺损的效果。方法将PLGA和胶原海绵制成直径4mm、厚3mm的圆柱形,分别与0.5m grhBMP-2复合。2月龄新西兰兔24只,雌雄不拘,体重1.8～2.3kg,平均2.0kg。于24只兔双侧股骨髁部制造直径4mm的缺损,其中18只动物右侧缺损处植入PLGA/rhBMP-2复合物(实验1组),左侧缺损处植入胶原海绵/rhBMP-2复合物(实验2组)?;余6只动物(12个膝关节)缺损不作任何处理,作为空白对照组。术后4、12和24周取材,行大体及组织学观察,并根据关节软骨半定量评分标准进行组织学评分。结果大体及组织学观察:4周,实验1组缺损内被半透明组织填充;实验2组缺损未被新生组织填满,两组形成的软骨组织与周围界限明显,软骨细胞分布较均一,排列无方向性;对照组中形成少量纤维组织。12周,实验1组缺损内完全充填白色半透明新生软骨组织,与正常软骨界限不清;实验2组缺损内形成白色半透明软骨组织,与周围正常软骨界限仍可辨认;两组新生软骨厚度逐渐接近正常软骨厚度,表面细胞平行排列,深层细胞排列较紊乱,基质异染,软骨下骨及潮线恢复,与正常软骨连接良好;对照组缺损边缘及底部形成少量软骨细胞,分布不均匀,底部纤维组织不连续。24周,实验1组缺损内形成半透明新生软骨组织,与正常软骨界限消失;实验2组形成的新生软骨组织颜色、质地与12周接近;两组新生软骨与正常软骨厚度相近,表面平整,细胞排列均一,但较紊乱,基质异染,软骨下骨及潮线恢复,与正常软骨连接良好;对照组缺损底部形成一层纤维组织,不连续。组织学评分:实验1组、实验2组与对照组在各时间点比较,差异均有统计学意义(P<0.01);两个实验组12、24周与4周比较差异有统计学意义(P<0.01),但12周和24周比较差异无统计学意义(P>0.05);同一时间点两实验组间比较差异无统计
Objective To compare the effect of PLGA and collagen sponge combined with rhBMP-2 on repairing of articular cartilage defect in rabbits respectively. Methods PLGA and collagen sponge were made into cylinders which were 4 mm in diameter and 3 mm in thickness, and compounded with rhBMP-2 （0.5 mg）. Defect 4 mm in diameter were made in both of femoral condyles of 24 two-month-old New Zealand white rabbits. The defects in right 18 knees were treated with PLGA/rhBMP-2 composites （experimental group 1）, and the left 18 knees were treated with collagen sponge/rhBMP-2 composites （experimental group 2）, the other 12 knees were left untreated as control group. At 4, 12 and 24 weeks after operation, the animals were sacrificed and the newly formed tissues were observed macroscopically and microscopically, graded histologically and analyzed statistically. Results From the results of macroscopical and microscopical observation, in the experimental group 1, the defects were filled with smooth and translucent cartilage; while in the experimental group 2, the white translucent tissues did not fill the defects completely; and in the two experimental groups, the new cartilage tissues demarcated from the surrounding cartilage, chondrocytes distributed uniformly but without direction; a little fibrous tissue formed in the control group 4 weeks postoperatively. In the experimental group 1, the defects were filled completely with white, smooth and translucent cartilage tissue without clear limit with normal cartilage; while in the experimental group 2, white translucent tissues formed, the boundary still could be recognized; in the two experimental groups, the thickness was similar to that of the normal cartilage; the cells paralleled to articular surface in the surface layer, but in the deep layer, the cells distributed confusedly, the staining of matrix was positive but a little weak; subchondral bone and tide mark recovered and the new tissue finely incorporated with normal cartilage;however, in the control group, there
出处
《中国修复重建外科杂志》
CAS
CSCD
北大核心
2008年第2期148-152,共5页
Chinese Journal of Reparative and Reconstructive Surgery
