摘要
目的:为了提高融合蛋白白喉毒素DAB389-(Gly4Ser)2-α-促黑激素的表达量,研究工程菌发酵条件的最优化。方法:利用三角瓶模拟小剂量发酵,在不同的受体菌、培养基、培养温度、时间、pH值、诱导剂浓度、诱导时间和温度等因素变化下研究目的蛋白的表达量。结果:工程菌优化的发酵条件为:最佳表达菌种为大肠杆菌BL21(λDE3),最适宜的培养基为M9B2,适合的pH值为7.0～7.4,最佳诱导温度为30℃,诱导时间为6 h,诱导剂IPTG的最佳用量为1.4 mmol/L。结论:获得了DAB389-(Gly4Ser)2-α-促黑素细胞激素工程菌的最优化发酵条件,为中试放大提供了理论依据。
Objective： In order to research on the highest expression of recombinant diphtheria toxin DAB389-（Gly4Ser）2- α-melanophore-stimulating hormone, the optimezed fermentation conditions of recombinant bacteria were studied. Methods： The fermentation conditions were investigated on the expression of protein in shake flask scale under different bacterical lines, culture medium, tempreture, culture time, pH, inducing concentration, inducing time and inducing tempreture etc. Results： Based on the experiments, there were the optimezed fermentation conditions such as that bacterical lines was E. coli BL21（λDE3）, culture medium was M9B2, pH was adjusted 7.0-7.4, inducing tempreture and time was 30℃ and 6 h respectively, IPTG was 1.4 mmol/L. Conclusion： The results showed that the optimezed fermentation conditions were established, which supplied a good tool for the magnify of recombinant bacteria.
出处
《生物技术通讯》
CAS
2008年第1期55-58,共4页
Letters in Biotechnology
基金
吉林省教育厅资助课题
吉林农业大学科学研究启动基金项目
关键词
α-促黑激素
白喉毒素
融合蛋白
工程菌
发酵
条件优化
α-melanophore-stimulating hormone
diphtheria toxin
refused protein
recombinant bacteria
fermentation
optimized conditions
