摘要
河南郑州地区某养鸡场的三黄肉鸡出现精神沉郁,拉水样白色粪便,剖检可见法氏囊水肿、出血;肾脏出血肿大,有尿酸盐沉淀,呈花斑状;腿肌、胸肌有出血斑点;腺胃与肌胃交界处出血。经病毒分离、鸡胚接种、琼脂扩散试验和细胞接种试验,最后确诊为传染性法氏囊病。同时,采集病料,制备冰冻切片,建立一种检测病料中的IBDV免疫荧光检测方法。
A virus was isolated from the Sanhuang broiler, which was suspected to be infectious bursal disease virus. By AGP and inoculated into CEF identification,the virus was infectious bursal disease virus. An IF was established for detection of IBDV of the infected tissue. The result indicated that the bursa was the seriously infected, caecal tonsil; kidney and spleen were second-class infected. No positive cell was found in the thigh muscle.
出处
《河南农业科学》
CSCD
北大核心
2008年第1期99-101,共3页
Journal of Henan Agricultural Sciences
关键词
传染性法氏囊病毒
分离鉴定
免疫荧光
Infectious bursal disease virus （IBDV）
Isolation and identification
Immunoflures-cenee （IF）
