摘要
利用RT-PCR的方法从普通小麦-簇毛麦6VS／6AL易位系克隆到1个cyp基因，命名为Ta-Hv-cyp。该基因全长为599bp，编码1条171个氨基酸残基的多肽，具有保守的功能位点W128和胞质型CyP蛋白特有的插入序列KSGKPLH48-54。BLAST分析表明，推导的Ta-Hv-CyP蛋白分别与小麦、水稻、玉米、拟南芥的胞质型CyP具有98％，88％，85％和80％的氨基酸序列一致性。构建的进化树显示，推导的Ta-Hv-CyP蛋白与单子叶植物胞质型CyPs的亲缘关系较近。
A full-length cDNA of a cyclophilin gene （cyp）, designated as Ta-Hv-cyp, was isolated from wheat-Haynaldia villosa 6VS/6AL translocation line by RT-PCR method. The Ta-Hv-cyp cDNA was 599bp in length with an open reading frame （ORF）encoding 171 amino acid residues.The deduced Ta-Hv-CyP protein contained a conserved functional site W128 and a conserved 7-a-mino acid insertion KSGKPLH48-54 which is specific in plant cytosolic CyPs. BLAST analysis showed that tht deduced Ta-Hv-CyP peptide had 98% ,88% ,85% and 80% identity with the corresponding Cyp of wheat, rice, maize and Arabidopsis thaliana, respectively. And phylogenetic analysis indicated that Ta-Hv-CyP might share the same origin with cytosolic CyPs of monocots.
出处
《河南农业科学》
CSCD
北大核心
2008年第1期16-19,共4页
Journal of Henan Agricultural Sciences
基金
国家863计划项目(2004AA222140)
江苏大学高级专业人才科研启动基金项目(05JDG033)
