摘要
目的:通过研究印迹基因H19在少弱精子症中的印迹状态,探讨H19与精子发生障碍的相关性。方法:采用聚合酶链式反应与逆转录-聚合酶链式反应,结合限制性片段长度多态性分析法(RFLP),对36例异常精液和15例正常精液标本进行H19印迹状态检测。结果:15例正常精液没有发现印迹丢失,36例异常精液检测出3例印迹丢失,印迹丢失率为8.3%。结论:H19印迹丢失与精子发生障碍有关,可能影响精子的数量。
Objective： To study the imprinting status of H19 in oligospermia and asthenospermia and to investigate the correlation between H19 and spermatogenesiss disturbance. Methods： PCR-RFLP and RT- PCR-RFLP were used to detect the imprinting status of H19 in the abnormal（36 cases） and normal（15 cases） semen. Results： There was no loss ofimprintion in the normal semen. Loss occured of imprintion in 3 cases of the abnormal semen. The rate was 8. 3%. Conclusion： The loss of imprinting of H19 is related to spermatogenesiss disturbance. It perhaps affects the quantity of semen.
出处
《陕西医学杂志》
CAS
北大核心
2008年第1期47-49,共3页
Shaanxi Medical Journal
