摘要
目的:观察兔脑损伤后肌酸激酶同工酶(CK-MB)表达的改变及其意义。方法:取成年灰色家兔采用自由落体法制成兔脑损伤动物模型,分别于伤后不同时段抽取动物静脉血和枕大池CSF,分析CK-MB的表达情况;而后将动物断头处死,取受伤区域脑组织进行脑组织含水量的测定;切片镜下观察组织的微观改变。结果:伤后6、24、72和168h实验组动物血清及CSF中CK-MB明显高于对照组(P<0.05或<0.01)。实验组动物伤后3、6、24和72h可见损伤区神经细胞水肿明显,微血管密度降低,微血管内微血栓形成,168h水肿的神经细胞和微血管形态基本恢复正常。结论:脑损伤后血和CSF中CK-MB的表达上调,其表达和脑损伤相关联,提示CK-MB可能参与了脑水肿的调控过程,CK-MB测定可能成为判断病人脑损伤的程度和预后的方法。
Objective ： To determine CK-MB after cerebral injury in rabbit and analyze the expression and significance of increased CK-MB in serum and CSF, and the changes of microcirculation. Methods ： Cerebral injury rabbit model was made by free falling,CK-MB in serum and CSF was determined, and the expression. Then the animals were killed, the fluid in injured cerebral tissue was measured, and the injured cerebral tissue was observed by microscopy. Results.. The CK-MB in serum and CSF was significantly increased at 6, 24,72, 168 hour after injury in experiment groups compared with the control group（P〈0.05 or〈0.01）. There was edema of nerve cells, decreased micrangiums,and microthrombus in micrangium at 6, 24, 48 and 72 hour in experiment groups, but these changes disappeared at 168 hour after injury. Conclusion：Expression of CK-MB in serum and CSF is up-regulated after cerebral injury, the results suggest that cerebral edema may be regulated and controlled. Measurement of CK-MB in serum and CSF of patient with craniocerebral injuries may become a test for the laboratory diagnosis of the trauma severity and the prognosis.
出处
《陕西医学杂志》
CAS
北大核心
2008年第1期16-18,共3页
Shaanxi Medical Journal
基金
河北省邯郸市科委资助课题[邯郸(2001)36-01079-3]
关键词
脑损伤/血液
肌酸激酶/代谢
同工酶类/代谢
脑损伤/病理学
微循环模型
动物
兔
Brain injury/blood Creatine kinase/metabolism Isoenzymes/ metabolism Brain injury/pathology Microcirculation Models ,animal Rabbit
