摘要
目的监测本地区2000-2006年临床细菌的构成分布及耐药性,并检测其是否产耐甲氧西林金黄色葡萄球菌(MRS)、耐甲氧西林凝固酶阴性葡萄球菌(MRCNS)、超广谱β-内酰胺酶(ESBLs)。方法MicroSCANAUTOSCAN4半自动鉴定药敏仪对送检标本进行菌种鉴定和药敏试验,琼脂筛选法检测MRS、MRCNS,K-B纸片扩散法检测ESBLs,比较产酶株与非产酶株对抗菌药物的活性。结果8448份临床标本检出各种细菌2436株,检出率为28.84%,种类以革兰阴性杆菌为主,占45.26%,最常检出的细菌依次为大肠埃希菌、CNS(-)葡萄球菌、肺炎克雷伯菌、铜绿假单胞菌、肠球菌属、金黄色葡萄球菌,MRS、MRCNS、ESBLs检出率高,耐药性强,耐药谱广。结论临床分离菌以条件致病菌为主,耐药株分离高,建议临床医生根据药敏试验合理用药,以减少耐药菌株产生。
Objective To monitor the local clinical bacteria distribution and their drug - resistance, and to detect MRS, MRCNS, and ESBLS in these clinical isolates. Methods Antimicrobial sensitivities were determined by Dade MicroSCAN AUTOSCAN4. MRS and MRCNS were detected with agar filtration method. ESBLs were detected with K - B susceptibility method. Results Among the 2,436 （28.84 % ） strains of germs isolated from 8,448 clinical samples, Gram - negative bacilli were the dominant pathogens （4.5.26 % ）. The pathogenic bacteria were Escherichia colia, and followed by Coagulase - negative staphylococous, Klelosiella pneumoniae, Pseudomonase aeruginosa, enterococcus and staphylococous. MRS, MRC- NS, and ESBLs showed high positive testing rate, and also had strong and wide drug- resistance spectrum. Conclusions Conditional pathogens are the major clinical bacteria and the drug - resistance is higher, therefore the clinicians should choose the suitable antibiotics according to the results of antibiotic susceptibility test for reducing the drug- resistance strain.
出处
《实用预防医学》
CAS
2007年第6期1729-1731,共3页
Practical Preventive Medicine
关键词
细菌
多重耐药
抗生素
Bacteria
Multiple drug resistance
Antibiotic
