摘要
目的:制备opticin特异性抗体并探讨opticin在牛眼中的分布。方法:将合成的多肽链免疫兔以获得OPT-C抗体,运用该抗体从玻璃体提取物中检测opticin,用免疫组织化学方法检测opticin在牛眼中的分布。结果:牛眼玻璃体提取物Western blot在40-50 kU可看到2个清晰可辨的分离的条带。牛眼免疫组织化学发现opticin存在于牛眼视网膜、玻璃体和睫状体无色素上皮中。结论:Opticin存在于睫状体中,支持opticin可能由睫状体无色素上皮细胞分泌这一推测。Opticin与玻璃体胶原纤维相连,可能与玻璃体结构的稳定有关。
Objective： To obtain specific antibody of opticin and to investigate the distribution of opticin in bovine eye. Methods： The distribution of opticin was detected from bovine vitreous extraction and paraffin embedded sections from bovine eyes were subjected to immunohistochemical analysis. Results： Western blot analysis of bovine vitreous specifically identified opticin as a discernible doublet at 40-50 kU. Opticin was immunolocalised to the retina, vitreous and ciliary body nonpigmented epithelium. Conclusion： Studies had previously demonstrated opticin expression by the non-pigmented ciliary epithelium. The immmunolocalisation data supported the proposition that the non-pigmented ciliary epithelium secrets opticin. The staining along the vitreous collagen suggests a connection between opticin and collagen, and opticin may be related to the stability of vitreous.
出处
《武汉大学学报（医学版）》
CAS
2008年第1期41-43,I0001,共4页
Medical Journal of Wuhan University
基金
武汉市晨光计划资助项目(编号:200750731255)
