摘要
在室内条件下,研究了降解菌S113(Methylopila sp.)对甲磺隆污染土壤的修复作用。S113能够以甲磺隆为唯一碳源生长,72h对50mgL-1甲磺隆的降解率达98.38%。投加降解菌S113可显著提高土壤中甲磺隆的降解速率。当甲磺隆浓度为10mgkg-1干土,S113接种量为108个g-1土时,第30天土壤中甲磺隆降解率为76.9%,对照土壤中甲磺隆降解率仅为11.9%。S113降解甲磺隆的速率和接种量呈正相关,当接种量减少为105个g-1干土时,降解菌对甲磺隆的降解作用微弱。在土壤中甲磺隆浓度较低的条件下,S113的降解效果显著,而当土壤中甲磺隆浓度达到50mgkg-1时,甲磺隆降解率仅为39.6%。S113降解土壤中甲磺隆的最适温度为30℃,第30天的降解率可达75.9%。当温度为25℃、20℃时,第30天甲磺隆降解率仅为53.5%和23.9%。S113菌剂灌根,能不同程度地缓解土壤中浓度为40、80μgkg-1的甲磺隆对玉米生长的抑制作用,但当甲磺隆浓度增加到120μgkg-1时,接种S113对药害解除作用不显著。结果表明,人工接种降解菌S113,能有效去除土壤中甲磺隆残留。
Bioremediation of metsulfuron-methyl contamined soil with S113 （ Methylopila sp. ） was studied under laboratory conditions. S113 was capable of utilizing metsulfuron-methyl as its sole carbon source for growth and degrading 98.38 % of the 50 mg L^-1metsulfuron-methyl within 72 h. Addition of S113 could prominently accelerate degrading of metsulfuron-methyl. When S113 was inoculated into the soil at a rate of 10^8 cells g^-1 dry soil, 76.9% of the metsulfuron-methyl （10 mg kg^-1 dry soil） in the soil was degraded after 30 days, whereas only 11.9% was degraded in CK. When inoculation rate decreased to 10^5 g^-1 dry soil, the action of S113 degrading metsulfuron-methyl in soil was not distinct, showing that degradation rate was related positively to the amount of inoculation, When metsulfuron-methyl was low in concentration, S113 was effective. When the concentration of metsulfuron-methyl was increased to 50 mg kg^-1, metsulfuron-methyl degradation rate was only 39.6%. Soil temperature also affected metsulfuron-methyl degradation rate. When it was 30℃ , the rate reached 75.9% in 30 days, whereas when it was 25℃ and 20℃ , the rate was lower to 53.5% and 23.9%, respectively. Pouring S113 solution around the roots with S113 solution could mitigate the inhibitive effect of metsulfuron-methyl at 40 or 80μg kg^- 1 on maize growth to a varying degree. When the concentration of metsulfuron-methyl increased to 120μg kg^-1, the mitigating effects were not distinct. The findings suggest that artificial inoculation of S113 could effectively degrade metsulfuron-methyl in soil.
出处
《土壤学报》
CAS
CSCD
北大核心
2008年第1期150-154,共5页
Acta Pedologica Sinica
基金
国家自然科学基金项目(30500010)
国家“863”计划项目(2006AA10Z402)
江苏省社会发展项目(BS2007056)
关键词
S113
甲磺隆
生物修复
S113
Metsulfuron-methyl
Bioremediation
