摘要
目的:探讨乙型肝炎病毒前S1抗原在诊断乙型肝炎病毒复制时的临床价值。方法:收集本院住院及门诊慢性乙型肝炎患者768例,用PCR定量分析法检测HBV-DNA,用酶免法(ELISA)检测前S1抗原,用时间分辨荧光免疫分析法检测乙型肝炎血清标志物。结果:以HBV-DNA≥103拷贝/ml判断病毒复制标准。在455例HBV-DNA阳性患者中前S1抗原阳性率为85.93%。在313例HBV-DNA阴性患者中,有81例前S1抗原阳性患者可能存在病毒复制,其原因有待进一步研究。HBV-DNA与前S1抗原两组阳性率结果比较,经χ2检验,χ2=282.350,P=0.000,差异有统计学意义。在455例HBV-DNA阳性患者中有123例HBeAg阴性患者存在病毒复制。HBV-DNA与HBeAg两组阳性率结果比较,经χ2检验,χ2=356.321,P=0.000,差异有统计学意义。在425例HBeAg阴性患者中,有210例前S1抗原阳性患者存在病毒复制,两组阳性率结果比较,经χ2检验,χ2=356.321,P=0.000,差异有统计学意义。结论:前S1抗原是诊断乙型肝炎病毒复制的重要指标。
Objective：The clinical value of hepatitis B virus pre-S1 antigen in the identification of virus replication was in-vestigated. Methods：A total of 768 chronic hepatitis B outpatients and inpafients have been collected. HBV -DNA was detected by the quantitative PCR analysis, HBV pre-S1 antigen by ELISA, and HBV serological marker by time-resolved immunofluoro-metric assay. Results：The virus replication standard was HBV-DNA≥10^3 copy/ml. Among the 455 HBV-DNA positive patients, the positive rate of pre-S1 antigen was 85.93%. Among the 313 HBV-DNA negative patients, virus replication may have occurred in 81 pre-S1 antigen positive patients, and the cause was to be further investigated. The positive rates of the HBV-DNA group and the pre-S1 antigen group were compared and there was significant difference by X^2 test （X^2=282.350, P=0.000）. Among the 455 HBV-DNA positive patients, virus replication has occurred in 123 HBeAg negative patients. The positive rates of the HBV-DNA group and the HBeAg group were compared and there was significant difference by X^2 test （X^2=356.321, P=0.000）.Among the 425 HBeAg negative patients, virus replication has occurred in 210 pre-S1 antigen positive patients. The positive rates of the two groups were compared and there was significant difference by X^2 test （X^2=356.321, P=0.000）. Conclusion：Pre-S1 antigen is a valuable marker for HBV replication.
出处
《中国卫生检验杂志》
CAS
2007年第12期2249-2250,2315,共3页
Chinese Journal of Health Laboratory Technology
