摘要
目的建立中药复智散提取物HPLC-UV/ELSD指纹图谱。方法采用HPLC-UV/ELSD串联检测技术,应用AlltechC18(5μm,4.6mm×250mm)色谱柱;流动相为乙腈(A)-0.1%乙酸水溶液(B),线性梯度时间洗脱程序(A)为:0,12%;25,20%;30,20%;75,30%;105,40%;120,80%;130,12%;流速:1.0ml/min;柱温:30℃;检测波长:335nm;飘移管温度80℃;氮气压力25psi。运用指纹图谱相似度评价软件,对12批复智散提取物指纹图谱进行相似度计算。结果建立中药复智散提取物指纹图谱并鉴定其5种主要成分;对UV与ELSD图谱互补性进行分析;12批复智散提取物指纹图谱相似度良好。结论该指纹图谱能同时反映复智散复方中主要化学组分特征,一次进样,可全面、稳定、有效地进行复方中药复智散的质量监督与评价。
Objective To establish a method for fingerprinting of Fuzhisan （FZS, a traditional Chinese medicinal preparation） using high-performance liquid chromatography with ultraviolet and evaporative light scattering detector （HPLC-UV/ELSD） to allow simultaneous determination of 5 major constituents in the preparation. Methods HPLC-UV/ELSD analysis was performed on water AlltechC18 column （5 um, 4.6 mm×250mm） with a mixture of acetonitrile （A） and 0.1% acetice acid water （B） as the mobile phase. The solvent A gradient for elution was 0, 12%; 25, 20%; 30, 20%; 75, 30%; 105, 40%; 120, 80%; 130, 12%, with the flow rate of 1.0 ml/min and the column temperature at 30℃. The detective wavelength was 335nm, drift tube temperature was 80℃, pressure of nebulizer gas was 25 psi. The similarities between the HPLC-UV/ELSD fingerprints of the 12 extracts were calculated using similarity evaluation software. Results The fingerprint of FZS was established and the 5 major constituents were identified. The complementarity between the fingerprints of UV and ELSD was analyzed, showing good correlation between 12 batches of FZS. Conclusions The method for fingerprinting can simultaneously characterize the main chemical constituents in FZS and allows stable, effective and comprehensive quality control and evaluation of FZS for a single sample.
出处
《南方医科大学学报》
CAS
CSCD
北大核心
2007年第12期1830-1833,共4页
Journal of Southern Medical University
基金
国家自然科学基金(30670726)
黑龙江省教育厅科学技术研究资助项目(11521137)~~
关键词
高效液相色谱
紫外/蒸发光散射检测
复智散
指纹图谱
high-performance liquid chromatography, ultraviolet
evaporative light scattering detection
Fuzhisan
fingerprinting
