摘要
目的研究放射对大鼠正常肝脏术后代偿再生能力影响及再生相关细胞因子表达的变化。方法雄性SD大鼠90只随机分为3个组，第1组全麻下行肝脏大部切除术（对照组），第2组行全肝4Gy照射后行肝脏大部切除术（4Gy组），第3组行全肝8Gy照射后行肝脏大部切除术（8Gy组）。术后分别在0．5、1．0、2．0、3．0、7．0、10．0d处死取残肝，通过残肝重量、有丝分裂指数、BrdU及PCNA标记指数比较各组肝脏再生能力；RTPCR和Western Blot比较各组再生相关细胞因子的变化。结果对照组术后1只腹部切口感染死亡，8Gy组2只大鼠放疗后胃肠道反应死亡。同对照组比4、8Gy组肝脏重量增长均变慢[第7天残肝平均重量分别为（10．474±0．41）、（9．49±0．83）、（7．214±0．23）g；t=3．15，P=0．021；t=2．46，P=0．014]，但12d时4Gy组恢复至对照组水平；同4Gy组相比8Gy组肝脏重量增长还慢（t=2．78，P=0．034）。8、4Gy组与对照组相比PCNA标记指数和BrdU掺入指数均有差别，最高值分别为55．3％±4．7％、71．2％±6．5％、80．2％±7．6％和25．3％±6．9％、35．3％±5．72％、44．2％±5．4％。术后第2天对照组比4、8Gy组有丝分裂指数高（t=2．04，P=0．031），4Gy比8Gy组也高（t=2．67，P=0．012）；分别为25．5％±4．6％、20．4％±2．7％、14．7％±3．8％。4Gy组术后HGFmRNA表达峰值较对照组下降，8Gy组峰值不明显。4、8Gy组p53及TGF-β1术后B表达高峰值比对照组高；TGF-βR Ⅱ对照组术后表达有下降趋势，4Gy组术后下降趋势不明显，8Gy组表现为持续增高。结论全肝照射抑制正常肝细胞潜在代偿增值能力，且呈剂量依赖性。
Objective To investigate the regenerative capacities and proliferation related cytokines expression of normal and irradiated livers following 70% partial hepatectomy in rats. Methods 90 male Sprague-Dawley rats were divided into 3 groups. The control group was only received 70% partial hepatectomy. The two irradiated groups were performed a single dose of 4 Gy and 8 Gy photon irradiation respectively followed 70% partial hepatectomy. The remanet liver were excised at 0.5,1.0,2.0,3.0,7.0 and 10.0 d after hepatectomy, and liver regeneration capacities in normal and irradiated rats were evaluated through remanet liver weigh, BrdU incorporation, PCNA labeling, and mitotic indices. The proliferation related cytokines expression were also compared. Results After radiotherapy and hepatectomy 1 rats died of infection in control group, and two rats in 8 Gy group died of radiation induced enteritis. The original liver weigh is regained within 12 days in control and 4 Gy group, and proliferative speed was lower in 4 Gy group than control group. The weigh of remanet liver in 8 Gy group was significantly lower than the two groups all time points from 2d. The apex of PCNA labeling index was 80.2 ±7.6% ,71.2 ±6.5% and 55.3 ±4.7% in control,4 Gy and 8 Gy groups respectively at 2d, and 44.2 ±5.4% ,35.3 ±5.72% and 5.3 ±6.9% with BrdU incorporation index. HGF mRNA expression peak of 4 Gy group was significantly lower than in control at 1 d, but no apparent peak value in 8 Gy group. TGF-β1 and TGF-βR Ⅱ level of radiation groups was apparently higher than the control group at different time points. Meanwhile the p53 protein tend to increase at 1 d then decline with high expression in irradiated groups. Conclusion Whole radiation can significantly inhibit the regenerative capacities following partial hepatectomy in rats with dose dependent
出处
《中华放射肿瘤学杂志》
CSCD
北大核心
2008年第1期55-59,共5页
Chinese Journal of Radiation Oncology
关键词
肝脏/放射
肝脏/手术
再生能力
大鼠
Liver/irradiation
Liver/operation
Regenerative capacity
Rat
