摘要
根据SAG1基因序列,自行设计一对寡核苷酸引物,利用PCR技术从弓形虫RH株基因组DNA中成功扩增出编码SAG1抗原的基因片段,扩增出的基因片段大小与预期长度(1006bp)相符,结果经测序验证,并利用生物信息学方法对SAG1蛋白理化性质、结构和功能进行了预测.
A pair of primers were designed and synthesized according to SAG1 gene sequence. The gene fragment encoding SAG1 antigen was successfully amplified from the genomie DNA of T. gondii RH strain by PCR technique. The size of gene fragment was 1 006 bp and in accordance with the target gene. The result was verified by sequence determination. Using bioinformatics analysis software, the physicochemical characteristics, structure and function of SAG1 protein was predicted.
出处
《生命科学研究》
CAS
CSCD
2007年第4期348-351,共4页
Life Science Research
关键词
弓形虫
SAG1基因
体外扩增
生物信息学分析
Toxoplasma gondii
SA G 1 gene
amplification in vitro
bioinformatics analysis