摘要
目的：观察维拉帕米在应激反应的抗氧化能力。方法：应用冷水浸头模拟应激刺激，将２２只小白鼠随机分为四组，组Ⅰ、组Ⅱ组为对照组：应激前腹腔内不注生理盐水和注生理盐水。组Ⅲ、组Ⅳ为实验组：应激前分别注维拉帕米１ｍｇ／ｋｇ和２ｍｇ／ｋｇ，检测心肌组织中的脂质过氧化物（ＬＰＯ）和谷胱甘肽过氧化物酶（ＧＳＨ－Ｐｘ）的含量。结果：与组Ⅰ及组Ⅱ相比，组Ⅲ的心肌ＭＤＡ及ＧＳＨ－Ｐｘ含量无显著改变（Ｐ＞０．０５），而组Ⅳ则显著降低，与组Ⅲ相比，组Ⅳ的心肌ＭＤＡ及ＧＳＨ－Ｐｘ水平明显降低。结论：２ｍｇ／ｋｇ维拉帕米在应激反应时可减轻氧自由基对小鼠心肌的损伤。
Objective:To observe the protective effects of verapamil on myocardial lipid peroxides reaction during stress.Method:Twenty two mices were randomly divided into four groups:Group Ⅰ:simple control without any administration,Group Ⅱ:the control with intraperitoneal normal saline before stress stimulation.Group Ⅲ and Ⅳ:receiving intraperitoneal verapamil 1 mg/kg and 2 mg/kg before stress stimulation respectively.The stress response was induced with the mice head being forcedly submerged into cold water of 4℃ in 1 min,10 min later,the myocardial sample was taken to measure the contents of MDA and GSH Px.Result:As compared with those of Group Ⅰ and Ⅱ,the mycardial levels of MDA and GSH Px unchanged significantly (P>0.05) in Group Ⅲ but decreased markedly in Group Ⅳ(P<0.05).Both levels were lower dramatically in Group Ⅳthan those in Group Ⅲ (P<0.05).Conclusion:Intraperitoneal verapamil at the dose of 2 mg/kg can effectively protect mice myocardium from the oxygen free radical during stress response.
出处
《中华麻醉学杂志》
CAS
CSCD
北大核心
1997年第7期433-434,共2页
Chinese Journal of Anesthesiology
