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十二烷基聚氧乙烯醚降解菌的分离、鉴定及降解特性 被引量:3

Isolation,identification of a alkyl-polyoxyethylene degrading bacterium strain and its degradation characteristics.
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摘要 从工厂排污口废水中分离.纯化并筛选出一株降解十二烷基聚氧乙烯醚(Brij-30)的菌株,鉴定为伯克氏菌属(Pandoraca sp),命名为B30.当温度为30℃,pH5~8,底物浓度低于0.2g/L,且以蛋白胨做氮源时,菌株B30降解效果最好;Zn^2+Ca^2+、Al^3+、Fe^3+对菌株B30的生长及其降解性能具有较大的促进作用.在低浓度区(0.05-0.20g/L),菌株B30生长最快,降解活力性最强,12h内能将底物基本降解;在中等浓度区(0.40g,L左右),72h内能降解50%的底物;在高浓度区(0.80-1.50g/L),72h内底物的降解率在30%以下. A bacterial strain B30 of degrading 12 alkyl-polyoxyethylene (Brij-30) was isolated, purified and sorted from the wastewater in pollution-outlets of a factory, and identified as Pandoraea sp.. At temperature of 30℃, pH of 5-8, Brij-30 concentration lowered 0.20g/L, protein peptone as the nitrogen source, bacterial strain B30 degradation effect was the best. Zn^2+, Ca^2+, Al^3+, Fe^3+ had greater promoting effect on the growth of bacterial strain B30 and its degradation property. At low concentration area (0.05-0.20g/L), bacterial strain B30 grew the quickest, degradation activity was the strongest, could degrade basically Brij-30-in 12h; in medium concentration area (about 0.40g/L), could degradate 50% Brij-30 in 72h; in high concentration area (0.80-1.50g/L), the degradation rate in 72h was lower under 30%.
出处 《中国环境科学》 EI CAS CSCD 北大核心 2007年第6期768-772,共5页 China Environmental Science
基金 国家"863"项目(2001AA115240) 湖南省科技攻关资助项目(05SK3097.06SK3060) 湖南省环境保护局重大资助专项(湘财建指[2004]90号)
关键词 筛选 16S RDNA 降解特性 十二烷基聚氧乙烯醚 screening 16SrDNA degrading characteristics alkyl-polyoxyethylene
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