摘要
目的:探讨地尔硫卓对大鼠急性心肌梗死(AMI)后心室重塑和心肌炎症反应的影响。方法:建立大鼠AMI模型,存活鼠随机分为地尔硫卓组(D组)和对照组(AMI组),另设假手术组(S组)。分别在梗死后1天、3天、1周、2周和4周测量各组大鼠超声心动图变化、HE染色检测心肌炎症细胞浸润及半定量RT-PCR检测炎症因子的表达。结果:与S组比较,AMI组射血分数下降和左室重量增加,心肌组织炎症细胞浸润,致炎因子TNF-α、IL-1β、IL-6表达增多(P<0.05),而D组射血分数和左室重量明显改善,心肌组织炎症细胞浸润减少,致炎因子表达减少(P<0.05)。结论:地尔硫卓治疗AMI可以有效改善左心功能和心室重塑,其机制可能与减弱心肌炎症细胞浸润和致炎因子表达有关。
Objective： To explore the effects of dihiazem on ventricular remodeling and inflammation in rat heart following acute myocardial infarction（AMI）. Methods： The model of AMI rats was randomly divided into diltiazem group（ D group）and control group （AMI group）,besides another group of sham operation（ S group）. The data of ejection fraction（EF） and the left ventricular mass （LVM） were examined with echocardiography, and leukocyte infiltration in situ was analyzed on the HE staining slices, with the expression of proinflammatory cytokines（ IL-1β, IL-6 ,TNF-α） detected by RT-PCR at 1 d ,3d, lw,2w and 4w intervals after AMI. Results ：The results from echocardiography showed that EF increased（73. 7 ±3.1% vs 61.0 ±2. 6% ）and LVM decreased（0. 81 ±0. 12g vs 0. 92 ± 0. 12g）, both significantly in D group at 4w, compared with those of the AMI group （P 〈 0. 05 ）. Leukocyte infiltration and the expression of IL-1β, IL-6, and TNF-α were remarkably lowered in D group（ P 〈 0. 05 ）, of which IL-1β was 1. 578 ± 0. 031 vs 0. 546 ± 0. 029 at 4w, IL-6 was 0. 952 ± 0. 012 vs 0. 532 ± 0. 011 at 2w, and TNF-α was 1. 001 ± 0. 006 vs 0. 526 ± 0. 009 at 4w. Conclusion ： Diltiazem could improve the left ventrieular function and attenuate ventrieular remodeling within AMI partly for the mechanism that diltiazem might influence the process of leukocyte infitration and the expression of proinflammatory eytokines.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2007年第7期653-655,共3页
Chinese Journal of Immunology
基金
国家自然科学基金资助项目(30600234
30370574)
关键词
急性心肌梗死
心室重塑
地尔硫卓
细胞因子
Acute myocardial infarction
Ventricular remodeling
Dihiazem
Cytokine
