摘要
以口蹄疫病毒株OA/58 RNA为模板,反转录并扩增目的cDNA,然后与pGEM-TEasy载体连接并转化JM109菌株,提取的重组质粒用凝胶电泳、PCR和EcoRⅠ酶切法鉴定。该毒株与Poliovirus,Hepatitis Avirus和Human rhi-novirus 89毒株3C序列对比分析发现核苷酸序列一致性分别为38.8%,37.1%和36.5%,氨基酸序列一致性分别为23.7%,19.2%和17.6%。通过Swiss-pdbViewer软件模拟出FMDVOA/58 3C蛋白酶的3D结构和表面模型。并鉴定出此毒株3C蛋白酶的活性中心为Cys31-His46-Asp84。
Foot-and-mouth disease virus strain OA/58 RNAs were used as templates for RT-PCR. The amplified cDNA products were cloned into pGEM-T Easy Vectors and transformed into JM109. The recombinant plasmids were identified by electrophoresis, PCR, and EcoR I cleavage. The nucleotide and amino acid sequences were compared with the 3C sequences of poliovirus, Hepatitis A virus and Human rhinovirus 89. The sequences alignments revealed that the agree- ments of OA/58 3C sequence to three reference viruses are 38.8%, 37.1%, 36.5 % at the nucleotide level, 23.7%, 19.2% ,17.6% at the amino acid level. By means of Swiss-pdbViewer software, the 3D and surface mold of FMDV OA/58 3C protease was obtained. In addition, Cys31-His46-Asp84may be an active site in FMDV OA/58 3C protease.
出处
《华北农学报》
CSCD
北大核心
2007年第6期9-13,共5页
Acta Agriculturae Boreali-Sinica
基金
国家重点基础研究发展计划"973"项目(2005CB523201)
