摘要
目的:探讨感毒清口崩片对流感病毒FM1致小鼠病毒性肺炎的细胞免疫作用机理。方法:以小鼠经鼻吸入流感病毒亚洲甲型鼠肺适应株(FM1)复制小鼠病毒性肺炎模型,用感毒清高、中、低剂量灌胃治疗5天,并设利巴韦林、莲花清瘟阳性对照及正常对照。采用SABC免疫组织化学法检测流感病毒感染小鼠肺组织细胞因子ICAM-1、NF-κB P65的表达。结果:感毒清高、中、低剂量组和利巴韦林、莲花清瘟组与模型组比较ICAM-1阳性表达明显减少(P<0.01);模型组NF-κB P65显著高于正常组(P<0.01)。各给药组NF-κB P65的活化程度(棕黄色颗粒)明显少于模型组(P<0.01)。结论:感毒清能抑制ICAM-1和NF-κB P65,从而抑制过度的炎症反应,减轻肺损伤。
Objective： To study the effects of Ganduqing buccal tablets （GDQ） on the mice with viral pneumonia infected by influenza virus FM1, and to explore the mechanism of cytoimmunity. Methods： Mice were inoculated intranasally with mouse lung-adapted influenza virus strain （FM1 ）, and were treated with GDQ in high dose, middle dose and low dose for five days. Ribavifin and Lianhua Qingwen （ a kind of TCM decoction ） were used as positive control and blank drugs respectively. SABC immunohistochemical method was used to detect the expression of cytokine ICAM-1, NF-κB P65 in paraffin section of lung tissue of mice infected by influenza virus FM1. Results： The expression of ICAM-1 in GDQ group （including high-dose group, middle-dose group and low-dose group） was significantly lower than that in Ribavifin group, Lianhua Qingwen group and model group （ P 〈 0.001 ） . The expression of NF-κB P65 in model group was significantly higher than that in the blank group （ P 〈 0.01 ） . The activation level （ dark yellow beads） of NF-κB P65 in GDQ groups, Ribavirin group and Lianhua Qingwen group was lower than that in the model group （ P 〈 0.01 ） . Conclusion： GDQ can restrain the expression of ICAM-1 and NF-κB P65, restrain the excessive inflammatory reaction and finally lessen the lung injury.
出处
《四川中医》
北大核心
2007年第12期20-22,共3页
Journal of Sichuan of Traditional Chinese Medicine
关键词
感毒清
流感病毒
细胞间粘附因子
细胞转录复制因子
Ganduqing buccal tablets （ GDQ） influenza virus intercellular adhesion molecule transcription and replication factors of cell
