摘要
建立了胶束电动毛细管色谱分离和测定蛇床子及其制剂中3种香豆素类活性组分的方法.系统考察了背景电解质pH、表面活性剂浓度、有机改性剂种类和浓度对分离的影响.实验结果表明:在缓冲液浓度为10 mmol/L、缓冲液pH为10.5、SDS浓度为20 mmol/L、甲醇浓度为10%时的优化条件下,蛇床子及其制剂中3种香豆素类活性组分得到基线分离.且方法具有较好的重现性.
A micellar electrokinetic capillary electrophoresis （MEKC） method was developed for the determination of three pharmaceutically important coumarins： osthole （OS）, imperatorin （IM） and isoimperatorin （IS） in Cnidium monnieri （L.） Cusson and its medicinal preparation （capsule of ‘Sanwushenwei＇）. A buffer solution （pH 10.50） composed of 10 mmol/L sodium borate, 20 mmol/L sodium dodecyl sulfate （SDS） and 10% methanol was found to be the most suitable electrolyte for this separation. The temperature and the applied voltage were 25 ℃ and 25 kV, respectively. Regression equations revealed linear relationships （correlation coefficients： 0. 999 8, 0. 999 4 and 0. 998 9） between the peak area of each compound （OS, IM and IS） and its concentration. The relative standard deviations of migration times and peak areas were 〈1.86 and 3.82% within one day, respectively. The effects of pH value, surfaetant （SDS） concentration and organic modifier on the migration behavior were also studied. By this way, the contents of active coumarins in the extracts of the Cnidium monnieri . Cusson and its medicinal preparation were successfully determined within 8 min.
出处
《分析测试技术与仪器》
CAS
2007年第4期272-276,共5页
Analysis and Testing Technology and Instruments
关键词
香豆素
蛇床子
毛细管胶束电动色谱
coumarin
osthole
micellar electrokinetic capillary electrophoresis
