摘要
用高效波相凝胶柱测定了云芝糖肽（PSP）主要组分的分子量，发现分子量随着流动相离子浓度的变化而变化，离子浓度越小，分子量越大。结果表明，以已知分子量的多糖Dextran作标准测得的云艺糖肽的分子量只是一个相对值，但在流动相离子浓度一定情况下仍可作为质量控制的一个指标。
High performance gel permeation chromatography (HPGPC) has been widely used in the fraction and molecular-weight (MW) estimation of polysaccharides. In this paper, we estimated the MW of major component of polysaccharide-pep tide from Corilous versicolor (PSP). These three-components were separated by using two column system (TSK-40 and TSK-50 gel columns) and different NaCl concentration in mobile phase. Calibration curve was prepared from the known MW Dextran T-system. It was found that the MW of PSP was changed in accordance with ion concentration of mobile phase. The MWs of the main component are more than 500 000, 120 000-19 000 and less than 10 000 respectively as the NaCl concentrations in mobile phase were less than 0. 002, 0. 006-0- 04 and more than 0. 06mol/L. The results showed that the MW of polysaccharide-peptide determined by HPGPC is a relative value that is changed with the concentration of mobile phase. The reason is that the molecular configuration and size of polysaccharide-peptide are changed along with electrolyte concentration in mobile phase. That is, the lower the electrolyte concentration is, the larger the molecular size of polysaccharide-peptide is, We suggest that it is s till useful for quality control of PSP as the mobile phase is fixed.
出处
《色谱》
CAS
CSCD
北大核心
1997年第2期150-152,共3页
Chinese Journal of Chromatography
关键词
云芝糖肽
糖肽
分子量
高效液相色谱
HPGPC
high performance gel permeation chromatography, polysaccharide - peptide, molecular-weight determination
