摘要
提出了一种液芯波导免疫分析阵列芯片的检测系统，结合光强差技术提高了ELISA的灵敏度，HRP的线性范围为1×10^-10～9×10^-10g／L，检出限为3．0×10^-11g／L，RSD小于1％（c=3×10^-10g／L，n=11），线性范围的下限与检出限均比普通的光度法下降了100倍，将其应用于血清和尿液中β-微球蛋白的免疫分析，与标准方法所得结果相符。
A detection system with liquid core waveguide array chip for immunoassay was put forward, which combined the technique of light intensity difference to enhance sensitivity of enzyme-linked immunosorbest assay （ELISA）. The linear rang for horseradish paroxidase （HRP） was 1 × 10^-10 g/L -9 × 10^-10 g/L with a detection limit of 3.0 × 10^-11 g/L. The RSD was less than 1% （c =3 × 10^-10 g/L,n = 11 ）, the linearity range low limit and detection limit were lower 100 times than usual spectrophotometry. This method was applied to detect β2-microglobin in serum and urine, and the result of sample analysis matched the standard method well.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
2007年第12期1776-1778,共3页
Chinese Journal of Analytical Chemistry
基金
国家自然科学基金资助项目(No.30470886)
关键词
液芯波导
酶联免疫
Β2-微球蛋白
Liquid core waveguide, enzyme-linked immunosorbest assay, β2-microglobin
