摘要
目的研究类风湿性关节炎(rheumatoid arthritis,RA)中葡萄糖-6-磷酸异构酶抗原(glucose-6-phospbate iso-merase,GPI)的检测及其临床意义。方法用ELISA方法分别对87例RA患者、40例其他自身免疫性疾病患者和72例正常人的血清进行GPI抗原检测;用免疫透射比浊法检测类风湿因子(rheumatoid factor,RF)。结果GPI抗原在RA中有59例阳性,阳性率为67.82%,在其他自身免疫性疾病中有1名,阳性率为2.50%,在正常人中全部阴性。RF在RA中有66名阳性,阳性率为75.86%,在其他自身免疫性疾病中有9例,阳性率为22.50%,在正常人中有8名,阳性率为11.11%。GPI抗原和RF对RA的特异性分别为99.11%和84.82%。GPI的敏感性与RF无明显差别(P>0.05),而特异性显著高于RF(P<0.01)。结论GPI抗原是检测RA的一项特异性很高的血清学指标,检测GPI抗原可能成为诊断RA患者的一个新的实验诊断指标。
Objective In this study, we aimed to explore the diagnostic value of glucose-6-phosphate isomerase （GPI） in patients with rheumatoid arthritis （RA）. Methods GPI were tested by an enzyme linked immunosorbent assay （ELISA） in serum samples from 87 patients with RA,40 patients with other autoimmune diseases, and 72 healthy individuals. Rheumatoid factor （RE） were assayed by turbidimetric immunoassay method. Results Amog 87 patients with RA, 59 patients showed positive of GPI（67.82% ） and 66 positiveof RF（75.86 % ）. Whereas, the positive percent was respectively 2.50 % and 22.50 % in 40 patients with other autoimmune diseases. In the health people, no patient was positive of GPI, but eight were positive of RF. The specificity of GPI and RF were 99.11% and 84.82%. The positive of GPI and RF was no significant association（ P 〉0.05）, but the specificity of GPI was significantly higher than the RF（ P 〈 0.01 ）. Conclusion GPI is a valuable diagnostic tool and a useful additional marker for RA. The detection of GPI is useful for the diagnosis of RA, especially combined with RF.
出处
《同济大学学报（医学版）》
CAS
2007年第6期105-108,共4页
Journal of Tongji University(Medical Science)
