摘要
目的探讨中药姜黄素对膀胱癌细胞(T24)增殖及凋亡的影响及可能机制。方法以培养的T24细胞为研究对象,姜黄素组加入终浓度为1、10、100μmol/L的姜黄素,对照组不加姜黄素,12、24、48h后观察T24细胞的形态变化,分别用MTT法、TUNEL染色、RT-PCR、免疫印迹及图像分析技术检测各组细胞生长、凋亡发生、AKT1蛋白及转录水平、caspase3蛋白含量的变化。结果姜黄素组T24细胞生长抑制率显著上升,细胞凋亡发生显著升高,AKT1蛋白活性水平降低,转录水平无显著变化,caspase3蛋白含量显著升高。结论姜黄素能抑制T24细胞的生长并促进其凋亡,其发生与其抑制T24细胞PKB/AKT信号通路有关。
Objective To investigate the effect and possible mechanism of curcumin on the proliferation and apoptosis of cell strain of bladder carcinoma. Methods In curcumin group, T24 cells were treated with curcumin of 1, 10, 100 μmol/L respectively, while no curcumin treatment in control group. The growth, apoptosis, AKT1 protein activity and mRNA level, caspase 3 content of T24 cells were observed by MTT, TUNEL, RT-PCR, immunoblot and image analysis technology 12, 24 and 48 h after the commencement of curcumin treatment. Results In the curcumin group, the inhibition rate, apoptosis and the content of caspase 3 of T24 ceils increased significantly. The activity of AKT1 protein decreased, but the mRNA level of AKT1 showed no changes. Conclusion Curcumin can inhibit the growth of T24 cells and induce the cell apoptosis, which mechanism is related to the inhibition of AKT1 signal pathway of T24 cells.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2007年第24期2350-2353,共4页
Journal of Third Military Medical University
关键词
姜黄素
膀胱癌
蛋白激酶B
curcumin
bladder carcinoma
protein kinase B
