摘要
目的研究高脂饮食诱导的大鼠非酒精性脂肪性肝炎形成中氧化应激/脂质过氧化的变化,观察山楂叶总酮对NASH的防治作用,探讨其作用机制。方法110只大鼠随机分为正常组40只、模型组40只、易善复组10只、山楂叶总黄酮高剂量及低剂量组各10只。正常组每天予以普通标准饲料喂养,其余各组每天均予以高脂饲料喂养;在造模的同时,易善复组灌服易善复混悬液195.4mg/(kg.d),山楂叶总黄酮高、低剂量组分别灌服山楂叶总黄酮250、125mg/(kg.d),正常组及模型组大鼠均灌服等量蒸馏水。实验开始后第2周、第4周、第8周末正常组及模型组分别随机处理大鼠10只,实验第12周末处理剩余所有大鼠。观察肝组织的病理改变,血清ALT、AST水平,肝组织匀浆TG、CHOL含量、血及肝匀浆MDA含量,SOD、GSH-PX、T-AOC的活力。结果(1)高脂饲料喂养2～12周,大鼠肝组织脂肪变程度逐渐加重,肝内TG、CHOL含量也逐渐增加;至12周肝组织炎细胞浸润明显,血清ALT、AST水平明显增高,呈现了NASH病变的渐进性发展过程。(2)高脂饲料喂养4周起血及肝匀浆MDA含量逐渐增高,SOD活力逐渐下降,肝组织匀浆的T-AOC也逐渐下降,且随炎症的进展而升高或降低。(3)山楂叶总黄酮高、低剂量组大鼠肝组织炎症程度、肝匀浆CHOL含量和血清ALT、AST水平较同期模型组明显减轻;血及肝组织MDA含量较同期模型大鼠显著减少,SOD、GSH-PX及T-AOC活性则较同期模型大鼠显著升高,且其抗氧化及减少脂质过氧化作用优于易善复。结论氧化应激/脂质过氧化在NASH的发生、发展中起重要作用。山楂叶总黄酮能有效防治NASH的发展,能明显缓解NASH大鼠的氧化应激,减少脂质过氧化,显著增强机体抗氧化能力,这是其防治NASH发生发展的重要作用机制。
Objective To study the change of oxidative stress in the formation of NASH in rats induced by high - fat diet, and observe the preventive and therapeutic effect of TFHL on NASH and explore its mechnism. Methods 110 rats were randomly divided into the normal group（40 rats） ,the model group（40 rats） , the A. Nattermam group（ 10 rats） and high dosage and low dosage TFHL （10rats each）. The normal rats were fed with the standard feedstuff the other rats fed with the high -fat diet. At the time of model - making, the rats in the A. Nattermam were fed the A. Nattermam suspension by daily gavage by 195.4mg/（ kg · d） ,the rats in the high dosage and low dosage TFHL group were fed TFHL by daily gavage in 250,125mg/（ kg · d） , the rats in the normal and model groups were fed isometrical distilled water by daily gavage. At the ends of 2nd, 4th ,8th weeks of the experiment , 10 rats in the nomal and model were killed respectively, at the end of experiment, all the rest were killed, pathological changes of liver tissues were observed, the levels of serum ALT, AST , the amount of TG, CHOL of livers tissue, the amount of MDA of serum and liver, the activity of OD,GSH - PX,T - AOC were measured. Resuits 1. the rats were fed with high - fat diet from 2 to 12w , the fatty degeneration of liver tissues were gradually serious,the amounts of TG, CHOL of livers were gradually increased, at 12^th w the inflammatory cells in livers infiltrated obviously, the serum ALT, AST were obviously increased,it apeared a progressive process of NASH. 2. from 4^th w,after feeding with high - fat diet,the amounts of MDA of serum and liver were gradually decreased, the activity of SOD were gradually increased, the amount of T - AOC were gradually decreased as well and, increase or decrease with the development of inflammation. 3. the inflammation degree of liver, the amount of liver CHOL and the serum ALT, AST in FMCL groups were lower than those in the corresponding model group; the amounts of MDA of serum and liver were decr
出处
《医学研究杂志》
2007年第12期33-36,共4页
Journal of Medical Research
基金
浙江省自然基金资助项目(Y2004310)
