摘要
固体培养基上比较了韩芝与树舌的菌丝生长速度，二者平均分别达到0．77cm·d^-1和0．67cm·d^-1；液体培养试验了不同碳氮比、培养基组成对韩芝菌丝生长的影响，得出了菌丝生长较适宜的碳氮比范围在18～24：1，最适宜的碳氮比在22：1；对比试验、正交试验得出韩芝液体培养最佳的培养基配方为（g·L^-1）：葡萄糖10．0、玉米粉20．0、大豆粉20．0、酵母粉5．0；在此条件下，菌丝的平均产量可达23．12g·L^-1。
The growth speed of Ganoderma lucidum HG and Ganoderma applanatumuan was compared, 0.770 cm· d^- 1 and 0.67 cm · d ^- 1 respectively. The effect of the ratio of C/N and the combination of medium were tested in liquid culture. The results showed that the better C/N was 20 - 24 ： 1, the perfect was 22 ： 1. The optimal fermentation medium were investigated by the contrast experiment and orthogonal devices. The optimum ingredient combination（g/L） ：glucose 10.0, corn powder 20.0, soya flour 20.0, yeast extract 5.0. In the conditions, the maximal biomass was 23.12g ·L/^-1
出处
《氨基酸和生物资源》
CAS
2007年第4期29-32,共4页
Amino Acids & Biotic Resources
基金
河南科技学院重点科研项目资助基金(5048)
关键词
韩芝
深层发酵
培养基
优化
Ganoderma lucidum HG
fermentation
medium
optimization
