摘要
合成一对猪Ⅱ型圆环病毒(PCV-2)ORF1基因特异性引物,对分离到的郑州分离株(ZZ株)猪圆环病毒PCV- 2型的ORF1基因进行了扩增,经测序后,对所测序列进行分析。结果显示,PCV-2 ORF1所表达的蛋白质具有良好的抗原性。
The ORF1 gene was amplified from ZZ strain of PCV-2 respectively using the primers designed according to the PCV- 20FR1 sequence published in Genbank. The nucleotide sequence data of PCV-20RF1 gene was analyzed, several conserved founctional domains were discovered in PCV-20RF1 gene and found the protein translated by PCV2-ORF1 gene having well immunogenicity.
出处
《郑州牧业工程高等专科学校学报》
2007年第4期1-3,共3页
Journal of Zhengzhou College of Animal Husbandry Engineering
基金
河南省科技发展计划项目(0621002000)
