摘要
对耐温黑根霉菌丝体催化16α,17α-环氧孕酮生成11α-羟基-16α,17α-环氧孕酮的反应体系进行了研究,考察了不同反应时间、不同菌体量和底物质量浓度对11α-羟化反应的影响,建立了相应的动力学模型,其方程为r=0.031 5ST1.05+ST。结果表明:提高菌体质量浓度有利于提高反应速率;底物浓度与反应初速率的关系与米氏方程相似。
A heat-resistant strain of Rhizopus nigricans was applied to the 11α-hydroxylation of 16α, 17α- epoxy progesterone to produce 11α-hydroxy-16α, 17α-epoxy progesterone. The effects of biomass, reacion time and substrate concentration on the reaction were investigated. The equation was r =0.031Sr/1.05＋Sr The results showed that the reaction rate was higher at higher cell concentration. The relationship between initial rate and substrate concentration can be correlated by the Michaelis-Menten equation and the Km =1.05 g/L,rm was 0. 031 g/（L·Ah）. The model was proposed to describe the initial rate of different substrate concentration.
出处
《生物加工过程》
CAS
CSCD
2007年第4期44-47,共4页
Chinese Journal of Bioprocess Engineering
基金
国家973资助项目(2003CB716008)
长江学者和创新团队发展计划资助项目(IRT0532)
