摘要
将不同浓度EMS处理的丽格海棠叶片作为外植体进行离体再生,以期获得性状优异的变异材料。结果表明:随EMS浓度的增加,其诱导率和增殖倍数降低,而变异率增加,0.4%(20℃,8h)EMS为丽格海棠叶片外植体再生的半抑制剂量,该浓度为诱变处理的适合浓度。对21株表型变异植株进行RAPD-PCR分析表明,有12株的电泳条带表现出多态性,进一步证明这些变异是由于基因组DNA的变异而引起的。
Leaf explants of Begonia x Rieger were regenerated and treated in vitro with chemical mutagen EMS at different concentration to get variation. The results showed that induction frequency and proliferation rate were reduced with the increase of EMS concentration, but the variation rate were increased. 0.4% EMS（8h, 20℃） was the half-lethal dose of the regeneration for leaf explants, and was the optimum concentration for variation occurrence of Begonia x Rieger in vitro. The 21 variant plants of Rieger x Begonia were detected by RAPD-PCR analysis, which showed that DNA polymorphism segments occurred in 12 variant plants, proving that came from genome.
出处
《核农学报》
CAS
CSCD
北大核心
2007年第6期577-580,596,共5页
Journal of Nuclear Agricultural Sciences
