摘要
应用聚丙烯酰胺凝胶(PAGE)垂直平板电泳对甜菜组织培养直接多芽发生和遗传转化过程中的过氧化物酶(POD)和酯酶(EST)同工酶动态及蛋白质含量的变化进行了研究。结果表明:甜菜在加入6-苄氨基嘌呤(6-BA)诱导直接多芽发生的过程中,POD、EST同工酶和可溶性蛋白出现了酶带条数和颜色深浅的变化,其变化可能与控制多芽发生的代谢活动有关;甜菜遗传转化后出现了明显的酶带颜色加深和新酶带的增加,这是由于外源基因的导入而使甜菜具有的标志,还是转基因的直接作用效果还有待于做进一步的研究。
The paper dealt with the study on the peroxidase and esterase isozymes as well as change of soluble protein content in the process of direct shoots organogenesis and genetic transformation of sugerbeet in vitro by polyarclamide gel electrophoresis（PAGE）. The results showed that peroxidase（POD）, isoesterase（EST）isoenzyme and soluble protein content appeared the changes of enzyme zymogram number and colour shade by adding benzylaminopurine（6- BA）to the medium, which related with increase of metabolizing activity. And after genetic transformation, bands of the peroxi- dase and esterase isozymes and soluble protein came forth obviously colour darken and number increase, which do far- ther research to identify the resons that resistance to stress was enhanced or natural transgene.
出处
《中国甜菜糖业》
2007年第4期9-13,共5页
China Beet & Sugar
基金
国家自然科学基金项目(30560080)
