摘要
目的探讨丙泊酚对布比卡因诱导的PC12细胞毒性的保护作用及活性氧(ROS)和过氧化氢酶(CAT)在其中的作用。方法培养的PC12细胞分成四组:正常对照组(C组);丙泊酚组(P组),在细胞培养基中加入2mmol/L丙泊酚;布比卡因组(B组),在细胞培养基中加入0.09mmol/L布比卡因;丙泊酚加布比卡因组(PB组),在细胞培养基中同时加入2mmol/L丙泊酚和0.09mmol/L布比卡因;每组6孔。培养6h和24h后,用倒置相差显微镜观察细胞形态、MTT比色微量分析细胞活性,测定上清液乳酸脱氢酶(LDH)活性和细胞内CAT、ROS活性。结果与C组相比,B组PC12细胞活性和细胞内CAT活性显著降低(P<0.01),LDH活性和细胞内ROS活性显著增加(P<0.01);P组PC12细胞活性及其它指标无显著变化;与B组相比,PB组PC12细胞活性和细胞内CAT活性显著增加(P<0.05),LDH活性和细胞内ROS活性显著降低(P<0.01)。结论布比卡因对PC12细胞具有毒性作用,可能与降低细胞内CAT活性、增加ROS活性有关;丙泊酚通过保护细胞内CAT活性和清除ROS而减轻布比卡因诱导的PC12细胞毒性。
Objective To investigate the protective effect of propofol on the bupivacaininduced cytotoxicity, reactive oxygen species （ROS） and catalase （CAT） in rat pheochromocytoma PC12 cells. Methods The PC12 cells were inoculated and cultured on the culture capsule. The cultured PC12 cells were randomly assigned to one of four groups with 6 holes each： control group （C）, propofol group（P）, bupivacaine group（B）, and propofol＋bupivacaine group（PB）. The viability of PC12 cells treated with different drugs for 6 h and 24 h was analyzed by MTT assay. The morphology of PC12 cells was observed under phase-contrast microscopy and photographed. Lactate dehydrogenase （LDH） in the culture medium was measured by spectroscopy. CAT and ROS activities in PC12 cells were assayed by spectrophotometer. Results Compared with normal PC12 cells, bupivacaine significantly reduced the relative viability （P〈0.01） and CAT activity of PC12 cells （P〈 0. 05）, and markedly increased LDH level in the culture medium and ROS activity in PC12 cells （P〈 0.01）. The relative viability and CAT activity of PC12 cells were significantly increased （P〈0. 05）, and the level of LDH in the culture medium and ROS activity in PC12 cells markedly decreased （P〈 0.01） in group PB compared with those in group B. Conclusion Bupivacalne induces cytotoxicity in PC12 cells line, which may be associated with ROS production and reduction of CAT activity. Propofol can prevent the bupivacaine-induced cytotoxicity of PC12 cells by protecting CAT activity and scavenging ROS production.
出处
《临床麻醉学杂志》
CAS
CSCD
2007年第11期912-914,共3页
Journal of Clinical Anesthesiology
基金
国家自然科学基金资助项目(NO30371763)
