摘要
目的评价环孢素A(CsA)壳聚糖纳米微粒抑制增生性玻璃体视网膜病变(PVR)的有效性和安全性。方法20只健康中华大耳白兔制作的PVR动物模型随机分为4组:A组玻璃体腔内注入0.1mL平衡液,B组玻璃体腔内注入0.1mL壳聚糖溶液(含壳聚糖2mg),C组玻璃体腔内注入0.1mLCsA溶液(含CsA0.1mg),D组玻璃体腔内注入0.1mLCsA壳聚糖纳米粒(含CsA0.1mg)。以检眼镜、电生理、光镜和电镜观察视网膜变化情况。结果玻璃体腔注药2周及4周后D组PVR等级均明显较A、B、C三组轻,组间差异具有统计学意义(P<0.05);而A组与B组、B组与C组之间比较差异无统计学意义(P>0.05)。D组注药前与注药后4周暗适应闪光ERGb波振幅的差异无统计学意义(P>0.05);D组注药后视网膜组织病理及超微结构未发现明显异常。结论一次性兔眼玻璃体腔注入CsA壳聚糖纳米微粒(含CsA0.1mg)能明显抑制或延缓PVR的发生,并具有生物相容性及安全性。
Objective This study was to evaluate the anti-proliferative effect and safety of cyclosporine A (CyA)-loaded chitosan (CS) nanoparticles in the treatment of proliferative vitreoretinopathy(PVR). Methods Platelet-rich plasma (PRP) was intravitreally injected to induce the experimental PVR in 20 white rabbits. The experimental rabbits were assigned to 4 groups. 0. 1 mL of BSS was injected into vitreous cavity in group A,and 0. 1 mL (2 mg) of CS solution was used in group B and CyA solution 0. 1 mL (CyA 0. 1 mg) in group C and CyA-loaded CS nanoparticles 0. 1 mL (CyA 0. 1 mg) in group D. After intravitreal injection, PVR was observed using ophthalmoscope at 1 day,3,7,14,21 and 28 days according to Fastenserg method. Scotopic flash ERG was recorded in the rabbits of group D preoperatively and 28 days after injection. Histopathological examination was performed in group D under the transmission electron microscope. Results The grading of PVR in group D was statistically lower than that in group A ( Z = 3. 724, P = 0. 000) , group B ( Z = 3.411, P = 0. 001 ) and group C ( Z = 3.180, P = 0. 002) on the 14th and 28th day, respectively( P 〈 0. 05 ). However, there was no significant difference in PVR grade between group A and group B(Z=1.594,P=0.110 1) or group B and group C(Z=1.681,P=0. 093).No any evident changes were found in b wave amplitude( 153.3 ± 17.9 μV in preinjection and 146.7 ± 19.2 μV in postinjection, t = - 0. 283, P = 0. 549 ) , histopathology or ultrastructure among various groups between before and after injection. Conclusion Intravitreal administration of CyA-loaded CS nanoparticles could predominantly inhibit the proliferative changes of experimental PVR induced by platelet-rich plasma. A dose of 0. 1 mg CyA-loaded CS nanoparticles is safe to retina of experimental rabbits.
出处
《眼科研究》
CSCD
北大核心
2007年第12期946-949,共4页
Chinese Ophthalmic Research