摘要
目的探讨乙肝相关疾病患者血清中IL-6及IL-8含量变化及其意义及IL-6、IL-8与乙肝病毒脱氧核糖核酸(HBV DNA)载量的相关性,寻找HBV感染的相关疾病的炎症活动指标。方法采用ELISA法检测慢性乙型肝炎(CHB)29例、乙肝后肝硬化(LC)31例、原发性肝癌(PHC)33例及正常对照30例血清中IL-6、IL-8的水平;并采用荧光定量聚合酶链反应(FQ-PCR)检测上述93例乙肝相关疾病患者血清HBV DNA的含量。结果CHB、LC及PHC患者血清IL-6、IL-8水平均显著高于对照组(P<0.01),但3组之间相比较无明显差异;HBV DNA阳性组和HBV DNA阴性组的IL-6、IL-8的含量均显著高于对照组(P<0.01),而且HBVDNA阳性组显著高于HBV DNA阴性组(P<0.01)。结论乙肝相关疾病患者存在着免疫调节的紊乱,IL-6、IL-8在其中起着重要的作用,而且IL-6、IL-8与HBV DNA载量存在正相关关系,可作为乙肝相关疾病炎症活动的观察指标。
Objective To study the level of IL-6,IL-8 and its significance in the serum of patients with hepatitis B virus-related diseases, and to explore the correlation among IL-6,IL-8 level and HBV DNA loads. Methods The levels of IL-6, IL-8 and HBV DNA loads were respectively detected by ELISA and FQ-PCR in serum in 93 cases,including 29 cases of CHB, 31 of LC, 33 of PHC and 30 of healthy people. Results The levels of IL-6, IL-8 in CHB, LC, and PHC groups were significantly higher than those in control group （P 〈 0.01 ） , without significant difference between CHB, LC,and PHC groups. The levels of IL-6, IL-8 in HBV DNA positive group and negative group were both significantly higher than those in control group （P 〈0.01）, while they were significantly higher in HBV DNA positive group than those in HBV DNA negative group （P 〈 0.01 ）. Conclusions The immuno-modulation function in patient with hepatitis B virus-related diseases are abnormal. IL-6 and IL-8 are involved in its pathogensis and have relationship with HBV DNA loads.
出处
《胃肠病学和肝病学杂志》
CAS
2007年第6期546-547,共2页
Chinese Journal of Gastroenterology and Hepatology
基金
广西卫生厅项目(Z2003126)
关键词
慢性乙型肝炎
原发性肝癌
乙肝病毒脱氧核糖核酸
白介素6
白介素8
Chronic hepatitis B （CHB）
Liver cirrhosis （LC）
Primary hepatocellular carcinoma （PHC）
HBV DNA
Interleukin-6 （IL-6）
Interleukin-8 （IL-8）
