摘要
目的：观察1，25二羟维生素D3对豚鼠实验性变态反应性脑脊髓炎（EAE）的预防和治疗效果方法：采用粗制碱性髓鞘蛋白（MBP）加福氏完全佐剂皮下注射的方法建立EAE模型。将30只豚鼠随机分成3组：正常对照组、EAE对照组、EAE治疗组，造模前3d正常对照组和EAE对照组每天灌喂0．4ml花生油，EAE治疗组分别在造模前3d开始灌喂含1，25二羟维生素D32．5μg．kg^-1．d^-1的花生油0．4ml，直至整个实验结束。观察豚鼠发病的潜伏期、进展期和神经功能症状；流式细胞仪法测定高峰期外周血T细胞亚群；酶联免疫吸附（ELISA）法测定高峰期外周血细胞因子IFN-γ、IL-4的含量。结果：正常对照组豚鼠未发病，EAE模型组豚鼠神经功能障碍明显。EAE治疗组与对照组相比，潜伏期明显延长，进展期明显缩短，发病高峰期神经功能障碍评分明显降低，死亡率明显降低；EAE对照组CD4^＋、CD8^＋含量比正常组降低，EAE治疗组CD4^＋、CD8^＋含量比EAE对照组高，EAE对照组CD4^＋／CD8^＋比值比正常组高，EAE治疗组CD4^＋／CD8^＋比值比EAE对照组降低；EAE对照组外周血单个核细胞分泌IL-4的能力较正常对照组明显降低，EAE治疗组外周血单个核细胞分泌IL-4的能力较EAE对照组明显增强。EAE对照组外周血单个核细胞分泌IFN-γ的能力较正常对照组明显增强，EAE治疗组外周血单个核细胞分泌IFN-γ的能力较EAE对照组明显减弱，其差异具有显著性。结论：1，25二羟维生素D3对EAE发病豚鼠具有保护作用：能延长EAE发病的潜伏期、缩短EAE发病的进展期、减轻神经功能障碍、降低高峰期死亡率；能提高外周血CD4^＋和CD8^＋含量、降低CD4^＋／CD8^＋比值。具有调节T细胞亚群失衡和纠正CD4^＋／CD8^＋比值倒置的作用；能抑制外周血单个核细胞分泌IFN-γ，促进外周血单个核细胞分泌IL-4，提示其具有抑制Th1细�
Objective To observe the protective effects of 1, 25-dihydrovitamin D3 on Guinea pig with EAEMethods We induced experimemtal allergic encephalomyelitis in Guinea pig by subcutaneous administrating coarse myelin basic protein （CMBP）. 30 Guinea pigs were included and were assigened randomly to three groups： namely, the normal group, the EAE contral group,the 1,25-dihydrovitamin D3treating EAE group.The normal group and EAE control group were perfused with the oil 0.4 ml from three days before the mould to intensive period of a disease, treating EAE group were perfused with the oil 0.4ml consisting of 1, 25-dihydrovitamin D3 2.5 μg/kg.d from three days before the mould to fastigium. The delitescence, the progression and the maximal disease score, mortality of each group were compared. The levels of T lymphocyte subclasses of peripheral blood in three groups were detected at fastigium with Flow CytoMeter （FCM）. The levels of cytokine IL-4 and IFN-r in peripheral blood mononuclear cells at fastigium by use of enzyme linked immunosorbent assay （ELISA）. Results There was no incidence of EAE in normal group. Comparing to the EAE control group, the delitescence of the treatment group was significantly longer （P〈0.05or P〈0.01）, the progression was significantly shorter （P〈0.05）, the death rate was significantly decline in treating EAE group （P〈0.05）. The percentage of CD4^＋ or CD8^＋T lymphocytes of peripheral blood in EAE control group significantly decreased in comparison with the normal group （P〈0.05）,the ratio of CD4^＋/CD8^＋markedly increased （P〈0.05）. The percentage of CD4^＋ or CD8^＋T lymphocyte in the peripheral blood of treating EAE group was markedly higher than those of EAE control group （P〈0.05）, but the ratio of CD4^＋/CD8^＋ was just the opposite （P〈0.01,P〈0. 05）. The levels of IL-4 in peripheral blood mononuclear cells in EAE control group were lower than those in the normal group（P〈0.01）.While in EAE control group, the levels of I
出处
《实用医技杂志》
2007年第24期3239-3243,共5页
Journal of Practical Medical Techniques
