摘要
目的:观察抗TNF-α单克隆抗体对豚鼠分泌性中耳炎的治疗效果。方法:建立分泌性中耳炎的动物模型,随机分成空白对照组、造模组和TNF-αMcAb治疗组三组。比较光镜和电镜下中耳、咽鼓管粘膜的组织形态及处理组TNF-α蛋白含量变化。结果:TNF-αMcAb治疗组中耳、咽鼓管粘膜的组织形态接近于正常对照组,其TNF-α蛋白含量明显低于造模组(p<0.001)。结论:抗TNF-α单克隆抗体能拮抗TNF-α的炎症介导作用,对豚鼠分泌性中耳炎治疗有效。
Objective： To observe the therapeutic efficacy of TNF - αMcAb in treating otisis media with effusion by being injected into tympanum in guinea pig. Methed： LPS was inoculated into the middle ear cavity in guinea pigs via a transeardrum approach to set up a model of otisis media with effusion, there was divided in three groups： control group, model group and TNF -αMcAb treating group. To observe the histo - morphological changes of eustachian tube and middle ear cavity and the content changes of TNF - α in treat groups. Result： The histo - morhological changes of TNF - αMcAb treating group is similar with control group, it＇ s content of TNF - otis significantly lower than model group （ p 〈0. 001 ）. Conclusion：TNF-αMcAb is efficient in treating otisis media with effusion in guinea pig via suppressing inflammatary introduction effect of TNF - α.
出处
《河北医学》
CAS
2007年第6期674-676,共3页
Hebei Medicine
关键词
中耳炎伴渗出液
肿瘤坏死因子-Α
单克隆抗体
动物模型
Otisis media with effusion
Tumor necrosis factor - alpha
Monoclonal antibody
Animal model
