摘要
目的探讨血管紧张素转换酶(ACE)基因多态性与老年人心脑血管病之间的相关性。方法用PCR、LDR及基因芯片技术对150例心脑血管病及126例正常对照组外周血白细胞作ACE基因多态性分析。结果ACED等位基因频率在老龄正常对照组及年轻对照组之间,在冠心病组与老龄正常对照组及年轻正常对照组之间均无显著性差别(P>0.05);高血压患者ACED/D基因型、脑梗死及脑萎缩组患者的ACED/D基因型与D等位基因频率均高于老龄正常对照组及年轻正常对照组(P<0.05与P<0.01)。结论ACED/D基因型可能是老年人高血压、脑梗死及脑萎缩的危险因素。
Objective To investigate the contribution of angiotensin converting enzyme （ACE） polymorphism in aged patients with heart and brain diseases. Methods All the 276 subjects （ 126 healthy controls,150 patients with heart and brain diseases） underwent the determination of ACE polymorphism from leukocyte DNA by polymerase chain reaction （PCR）, ligase detection reaction （LDR） and gene chip. Results For ACE D/D genotype, no significant difference was observed between aged and young healthy groups （P 〉 0.05 ）, aged or young healthy control group and coronary heart disease group （P 〉 0.05 ）, but difference was existed between essential hypertension group and aged or young healthy control group （P 〈 0.05 ）. Significant differences were also found between aged or young healthy control group and brain infarct group as well as brain atrophy group（P 〈0.01 ）. Conclusion ACE D/D genotype may be the risk factor of essential hypertension, brain infarct and brain atrophy.
出处
《临床检验杂志》
CAS
CSCD
北大核心
2007年第6期413-415,共3页
Chinese Journal of Clinical Laboratory Science
基金
江苏省临床免疫学重点实验室基金项目(NO200319)。
关键词
ACE基因多态性
冠心病
原发性高血压
脑梗死
脑萎缩
ACE polymorphism
coronary heart disease
brain infarct
essential hypertension
brain atrophy
