摘要
本研究探讨甲氧基聚乙二醇(mPEG)化学修饰移植物细胞对小鼠单倍体相合干细胞移植后移植物抗宿主病(GVHD)的影响。取经mPEG修饰及未修饰的成年CB6小鼠脾细胞悬液注入BALB/c新生幼鼠的腹腔(5×106脾细胞/只),以脾增大指标判断新生BALB/c小鼠注射单倍体相合供鼠脾细胞后的GVHD反应;取经mPEG修饰及未修饰的成年BALB/c小鼠骨髓及脾细胞混合悬液(BMS)尾静脉注入接受致死量γ射线照射后的成年BALB/c小鼠(2×105个BMS/只),注射后第8天取受鼠脾脏,计数脾结节数。结果表明:修饰组脾指数1(SI1)较未修饰组减低,修饰组脾指数2(SI2)值<1.3,未修饰组SI2值>1.3,提示接受mPEG修饰的单倍体相合脾细胞输注后,修饰组GVHD程度较未修饰组轻;未修饰组与修饰组相比较,脾结节形成的数量差异无显著性意义(p>0.05),说明mPEG修饰不影响造血干祖细胞的活性。结论:mPEG修饰移植物可减轻小鼠单倍体相合干细胞移植后的GVHD,而不影响干祖细胞活性。
This study was aimed to explore the effect of ex vivo chemical modification of graft cells with methoxy polyethylene glycol （mPEG） on graft versus host disease （GVHD） after haploidentical stem cell transplantation in neonatal mice and its influence on activity of the stem cells. The modified and non-modified spleen cells of adult CB6Ft mice were injected into the abdominal cavity of neonatal BALB/c mice with 5 × 10^6 spleen cells per mouse, and GVHD were measured by spleen index （SI）. Furthermore, the modified and non-modified mixture of bone marrow and spleen cells （BMS） were transplanted to haploidentical lethally irradiated adult BALB/c mice via tail vein with 2 × 10^5 BMS per mouse, and the colony forming units of spleens （CFU-S） were counted on the eighth day after irradiation. The results indicated that SIt in modification group were lower than that in non-modification group, and SI2 in modification group was 〈 1.3, showing that GVHD in modification group were less severe. The numbers of CFU-S formed in both modification group and nonmodification group were not significantly different （p 〉0.05）, indicating that the activity of the stem cells were not affected by mPEG modification. In conclusion, the modification of graft cells with mPEG alleviates GVHD after haploidentical stem cell transplantation in neonatal mice, and do not influence the activity of the stem cells.
出处
《中国实验血液学杂志》
CAS
CSCD
2007年第6期1240-1242,共3页
Journal of Experimental Hematology
