摘要
利用速生杨树(Populus.tomentosa Car)叶片为外植体建立离体培养体系,结果表明,叶脉处和叶柄处,极易发生不定芽;芽大多是从叶片上直接产生;培养基中BA浓度是影响不定芽形成的关键;BA浓度在05～2.0mg/L范围内均有不定芽发生,BA1.0mg/L与NAA0.05mg/L搭配有利于不定芽的形成,培养基中添加GA30.1～0.2mg/L可以提高不定芽的发生频率,增殖培养基采用MS+BA0.5mg/L+NAA0.05mg/L+GA30.05mg/L,不仅能够快速增殖,而且芽苗粗壮,玻化率降低。生根过程采用过渡培养效果较好,ABT3生根粉的加入,使试管苗质量大为提高,在此基础上建立起杨树叶片离体再生系统,利用该体系能够获得高的芽苗再生频率,但是不同品种间略有差异。
The leaves of fast growing poplar were used as explants and a in vitro culture system was established. The results showed that the leaf vein and petiole produced adventitious buds very easily and most buds occurred directly in the leaves; the BA concentration in media is the key to the bud formation; when the concentration was 0.5 ～2.0mg/L BA, the adventitious buds occurred, and combining 1.0mg/L BA with0.05mg/1 NAA was beneficial to the adventitious bud formation; adding 0.1 ～0.2mg/L GA3 to the media could increase the Frequency of producing the adventitious buds. Use the propagation medium MS＋0.5mg/L BA＋0.05mg/L NAA ＋0.05mg/L GA3 could not only realize rapid propagation but also make the plantlets robust and the verifications rate lower. Adopting transition culture in rooting had a better result, and adding ABT3-rooting-powder to the media made the quality of test-tuber plantlets much better. The in vitro regen- eration system of leaves of the poplar was established based on the experimental results. Using this system could reach a high regeneration frequency but different varieties would differ a little bit.
出处
《中国农学通报》
CSCD
2007年第12期88-92,共5页
Chinese Agricultural Science Bulletin
基金
安徽省省级课题"甘薯脱毒及脱毒苗快繁技术研究"项目(皖99541)。
关键词
杨树
叶片
培养基
离体再生体系
poplar, leaf, medium, in vitro regeneration system
