摘要
目的观察海洛因依赖对大鼠下颌下腺表皮生长因子(EGF)及白细胞介素2(IL-2)阳性细胞形态及功能的影响。方法正常SD大鼠,随机分为空白对照组、盐水对照组及海洛因依赖组。皮下注射海洛因建立海洛因依赖大鼠模型,分别于第10、17、24、31、38d取下颌下腺组织。用免疫组织化学SABC法、图像分析及细胞计数方法进行研究。结果1.与空白对照组相比,盐水对照组大鼠下颌下腺EGF、IL-2阳性细胞的数量及强度均无明显改变。2.海洛因依赖期间,大鼠下颌下腺EGF、IL-2阳性细胞的免疫反应增强,细胞数量明显增加(P<0.05)。图像分析结果显示,在整个海洛因依赖期间,EGF、IL-2阳性细胞的平均灰度值始终低于空白组及盐水对照组(P<0.05)。3.EGF与IL-2在大鼠下颌下腺内分泌细胞中有共存现象。结论在海洛因依赖期间,大鼠下颌下腺EGF、IL-2阳性细胞的数量及反应强度均发生了规律性改变,提示大鼠下颌下腺分泌的EGF及IL-2参与了机体对海洛因依赖的调节过程。
Objective To observe the effect of heroin on EGF and IL-2 positive cells in the submandibular gland of rats. Methods Normal SD rats were divided into normal control group （NGC）,saline control group （SCG） and heroin dependence group （ HDG）. The heroin dependent model of rats was established by subcutaneous injection of heroin, and submandibular glands were excised on the following days respectively： the lOth, 17th, 24th, 31th and 38th. Immunohistoehemical SABC method, image analysis and cells calculation were used in the study. Results 1. As compared with those of the NCG, the number of EGF and IL-2 positive cells and the intensity of staining in SCG didn＇ t change obviously. 2. During heroin dependence, the immunohistoehemical staining showed that the number of EGF and IL-2 positive cells increased obviously（ P 〈 0.05 ） ; meanwhile, the results of image analysis showed that the mean grey degree of EGF and IL-2 positive cells were lower than that of NCG and SCG groups. 3.The co-localization of EGF and IL-2 was proved in the submandibular gland of rats. Conclusion The number of EGF and IL-2 positive cells and the intensity of staining change regularly in the submandibular gland of rots. It suggestes that the EGF and IL-2 from submandibular gland of rat were involved in the regulating process of heroin dependence.
出处
《解剖学报》
CAS
CSCD
北大核心
2007年第6期731-735,共5页
Acta Anatomica Sinica
基金
贵州省优秀科技教育人才省长专项基金资助项目(黔科教办2001-3)
教育部"春晖计划"资助项目(Z2005-1-52020)
