摘要
将猪传染性胃肠炎病毒（ＴＧＥＶ）Ａ株在ＩＢＲＳ２细胞上增殖，培养物经差速离心浓缩后与鸡红细胞出现了高凝集价（２８），这种凝集作用能被兔抗ＴＧＥＶ高免血清所抑制。试验的最适反应条件为体积分数０．５０％红细胞４℃作用１ｈ。通过反复试验，建立了一种简单实用的血凝抗原制备方法，病毒培养液不需浓缩可直接应用。
IBRS 2 Cells were infected by transmissible gastroenteritis virus strain A,and the culture fluid concentrated with differential centrifugation which and chicken erythrocytes showed high agglutination titer(256).The rabbit anti TGEV serum can inhibit the hemagglutination.The optimal physical condition for HA was that 0.5% erythrocytes were incubated With TGEV at 4℃ for 1 hour.With repeapted test,a simple and practical method of preparation hemagglutinavite antigen was established.
出处
《中国兽医科技》
CSCD
1997年第9期3-4,共2页
Chinese Journal of Veterinary Science and Technology
